Fluorescence resonance energy transfer (FRET) microscopy imaging of live cell protein localizations.
Author: Sekar RB, Periasamy A.
Affiliation:
W.M. Keck Center for Cellular Imaging, Department of Biology, Gilmer Hall (064), University of Virginia, Charlottesville, VA 22904, USA.
Conference/Journal: J Cell Biol.
Date published: 2003 Mar 3
Other:
Volume ID: 160 , Issue ID: 5 , Pages: 629-33 , Word Count: 60
The current advances in fluorescence microscopy, coupled with the development of new fluorescent probes, make fluorescence resonance energy transfer (FRET) a powerful technique for studying molecular interactions inside living cells with improved spatial (angstrom) and temporal (nanosecond) resolution, distance range, and sensitivity and a broader range of biological applications.
PMID: 12615908 [PubMed - indexed for MEDLINE] PMCID: PMC2173363 Free PMC Article
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