Fluorescence resonance energy transfer (FRET) microscopy imaging of live cell protein localizations. 


Author: Sekar RB, Periasamy A.

Affiliation: W.M. Keck Center for Cellular Imaging, Department of Biology, Gilmer Hall (064), University of Virginia, Charlottesville, VA 22904, USA. 
Conference/Journal: J Cell Biol.
Date published: 2003 Mar 3
Other:
 Volume ID: 160   ,  Issue ID: 5   ,  Pages: 629-33   ,  Word Count: 60  
    

The current advances in fluorescence microscopy, coupled with the development of new fluorescent probes, make fluorescence resonance energy transfer (FRET) a powerful technique for studying molecular interactions inside living cells with improved spatial (angstrom) and temporal (nanosecond) resolution, distance range, and sensitivity and a broader range of biological applications.

PMID: 12615908 [PubMed - indexed for MEDLINE] PMCID: PMC2173363 Free PMC Article