Pairs of Helix aspersa neurons show a magnetic field dependent frequency synchronization (AMFS) when exposed to weak (amplitude B0 between 0.2-150 Gauss (G)) alternating magnetic field (AMF) of extremely low frequency (ELF, fM=50Hz). We have compared the AMFS patterns of discharge with: i) the synaptic activity promoted by glutamate and acetylcholine; ii) the activity induced by caffeine; iii) the bioelectric activity induced on neurons interconnected by electric synapses. AMFS activity reveals several specific features: i) a tight coincidence in time of the pattern and frequency, f, of discharge; ii) it is induced in the time interval of field application; iii) it is dependent on the intensity of the sinusoidal applied magnetic field; iv) elicited biphasic responses (excitation followed by inhibition) run in parallel for the pair of neurons; v) some couple of neurons either spontaneously or AMF synchronized can be desynchronized under applied higher AMF. Our electron microscopy studies reveal gap junctions-like confirming our immunocytochemistry results about expression of connexin 26 (Cx26) in 4.7% of Helix neurons. AMF and carbenoxolone did not induce any significant effect on spontaneous synchronization through electric synapses.
© 2013.
KEYWORDS:
AMF, AMF sinusoidal frequency, AMFS, Ach, Bioelectromagnetism, CBX, Cx26, ELF, ELF-magnetic field effects, EMF, G, Glut, Inx, MF, Magnetobiology, Mollusc brain ganglia, N-methyl-D-aspartate, NMDA, Neuromagnetism, Pnx, RF, RN, Ringer solution, SAR, SD-CE, SMF, Synchronization of neuron activity, T, TEA, TEM, Tesla, W, acetylcholine, alternating magnetic field, bioelectric frequency (spikes/s≡s/s), caffeine, carbenoxolone, cf, connexin 26, e, electromagnetic field, electron charge, extremely low frequency, f, fM, gauss, glutamate, innexins, magnetic field, magnetic field dependent frequency synchronization, membrane bilayer superdiamagnetism and Ca(2+)-coulomb explosion, pannexin, radiofrequency, specific absorption rate, static magnetic field, tetraethylammonium, transmission electron microscopy, washing neuron bath with fresh Ringer solution
PMID: 24012769
