A novel acetylcholine bioensor and its electrochemical behavior.

Author: Wang W, Deng Y, Li S, Liu H, Lu Z, Zhang L, Lin L, Xu L.
Key Laboratory of Green Packaging and Application of Biological Nanotechnology, Hunan University of Technology, Zhuzhou 412007, China.
Conference/Journal: J Biomed Nanotechnol.
Date published: 2013 Apr
Other: Volume ID: 9 , Issue ID: 4 , Pages: 736-40 , Word Count: 84

A novel type of acetylcholine electrochemical biosensor was successfully fabricated by dropping beta-cyclodextrin solution, acetylcholinesterase solution and Nafion-methanol solution to the homemade nano-porous pseudo carbon paste electrode (nano-PPCPE) as working electrode, and then the electrochemical behavior of the as-prepared biosensor was studied through the acetylthiocholine iodide (ATChI). It was shown that the modified nano-PPCPE displays a high selectivity and sensitivity. The acetylcholine biosensor showed the linear range from 5 x 10(-6) M to 2 x 10(-4) M with the detection limit of 1.5 x 10(-7) M.
PMID: 23621036