Osteogenic differentiation of adipose-derived stem cells prompted by low-intensity pulsed ultrasound.

Author: Yue Y, Yang X, Wei X, Chen J, Fu N, Fu Y, Ba K, Li G, Yao Y, Liang C, Zhang J, Cai X, Wang M.
State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China; Department of Prosthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, 610041, China.
Conference/Journal: Cell Prolif.
Date published: 2013 Jun
Other: Volume ID: 46 , Issue ID: 3 , Pages: 320-7 , Special Notes: doi: 10.1111/cpr.12035. , Word Count: 167

Based on in vivo studies, low-intensity pulsed ultrasound (LIPUS) stimulation has been widely used in the clinic for advancing bone growth during healing of non-union alignment, fractures and other osseous defects. In this study, we have investigated osteogenic differentiation of adipose stem cells (ASCs) regulated by LIPUS, and also in a preliminarily manner, we have discussed diverse effects of different duty ratio parameters.
Mouse adipose stem cells were isolated and osteogenically induced. Then they were treated with LIPUS for 10 min/day for 3 days, 5 days and 7 days, respectively. Finally, effects of LIPUS on osteogenic differentiation of the ASCs were analysed by real-time PCR, western blotting and immunofluorescence.
Our data indicated that LIPUS promoted mRNA levels of runt-related transcription factor 2, osteopontin and osterix in the presence of osteo-induction medium; moreover, protein levels of runt-related transcription factor 2 and osteopontin were upregulated.
We successfully demonstrated that LIPUS enhanced osteogenesis of ASCs, specially at the duty ratio of 20%.
© 2013 Blackwell Publishing Ltd.
PMID: 23692090 [PubMed - in process]