DNA Integrity of Human Leukocytes after Magnetic Resonance Imaging.

ABSTRACT Purpose: This study focuses on the effects of high-field (3T) magnetic resonance imaging (MRI) scans on the DNA integrity of human leukocytes in vitro in order to validate the study where genotoxic effects were obtained and published by Lee et al (Lee at al. 2011). Materials and methods: The scanning protocol and exposure situation were the same as those used under routine clinical brain MRI scan. Peripheral blood samples from healthy non-smoking male donors were exposed to electromagnetic fields (EMF) produced by 3 T magnetic resonance imaging equipment for 0, 22, 45, 67, and 89 min during the scanning procedure. Samples of positive control were exposed to ionizing radiation (4 Gy of 60Co-γ). Single breaks of DNA in leukocytes were detected by single cell gel electrophoresis (comet assay). Chromosome breakage, chromosome loss and micronuclei formations were detected by a micronucleus test (MN). Three independent experiments were performed. Results: The data of comet tail DNA%, olive tail moment and micronucleus frequency showed no DNA damages due to MRI exposure. Conclusions: The results of the comet assay and the micronucleus test indicate that the applied exposure of MRI does not appear to produce breaks in the DNA and has no significant effect on DNA integrity.
PMID: 23679232 [PubMed - as supplied by publisher]