Low-intensity pulsed ultrasound-mediated stimulation of hematopoietic stem/progenitor cell viability, proliferation and differentiation in vitro.
Author: Xu P, Gul-Uludag H, Ang WT, Yang X, Huang M, Marquez-Curtis L, McGann L, Janowska-Wieczorek A, Xing J, Swanson E, Chen J.
Affiliation:
ECERF W6-019, Department of Biomedical Engineering, University of Alberta, Edmonton, AB, T6G 2V4, Canada, px1@ualberta.ca.
Conference/Journal: Biotechnol Lett.
Date published: 2012 Jul 5
Other:
Word Count: 117
Low-intensity pulsed ultrasound (LIPUS) stimulated the viability, proliferation and differentiation of hematopoietic stem/progenitor cells (HSPC) from fresh and cryopreserved peripheral blood leukapheresis product, as well as cord blood when applied for 10 min each day for 4 days. Cell viability, proliferation and differentiation were assessed on day 5 by viable cell counting, MTS proliferation assay, flow cytometry, and colony-forming unit assay. LIPUS stimulation: (i) enhanced the proliferation of fresh HSPC and maintained the viability of cryopreserved HSPC in vitro; (ii) did not affect the percentage of CD34(+) and CD14(+) cells; and (iii) enhanced burst-forming unit-erythroid colony formation. Hence, we suggest that this novel LIPUS stimulation approach might enhance the efficacy of clinical transplantation and cellular therapies using HSPC.
PMID: 22763849
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