Analysis of effect of emitted qi on human hepatocarcinoma cell (BEL-7402) by using flow cytometry

Based on our previous studies of the cellular effect of the emitted qi on human hepatocar cinoma cell (BEL-7402) in this experiment, the state-of-the-art technique of flow cytometry (FCM) was employed to analyze the influence of it on BEL-7402 from the angles of cell division, kinetics and genetics.

Repeated results indicated that the emitted qi treatment inhibited the higher rate of DNA synthesis in BEL-7402 and decreased the concentration of DNA . Meanwhile, the cell cycle of BEL-7402 was correspondingly altered. GO, G1, G2 phases became more obvious by repeated treatments, but S phase appeared to be smaller. After the emitted qi treatment, chromosome karyotype changed too. These results demonstrated that the BEL-7402 cell could be restored to the normal to some extent by qigong action. For the further confirmation, naked mice were inoculated with BEL-7402 and the treated cell, respectively, and it was surprisingly observed that the tumor was only induced in the naked mice inoculated with BEL-7402, whereas naked mice with qigong treated BEL-7402 grew well. This result was further evidenced by the FCM analysis of cells isolated from in situ tissue.