We used the techniques of tissue culture, cytogenetics and electron microscopy to study the Hale cells and SGC-7901 human gastradenocarcinoma cells, the change of the cellular ultrastructure and abnormal structure of the chromosomes of the cells. The results showed
1. The effect of the emitted qigong on the Hale cells:
We repeated the experiment 20 times under identical conditions. The result is shown in Table 1.
Table 1. The effect of the emitted qi on the Hale cells
Number of the Hale cells (10/ml) Survival Destruction
Group Before qi After qi rate rate
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Exp. 4.53 15.33 100.00 O.0
Control 4.53 10.62 69.28 30.72
Table 1 shows, after the Hale cells received the emitted Qi for 20 minutes, the survival rate of the cells in the experiment group was 69.28% of that in the control group, i. e., 30.72% of the cells were killed.
We analyzed the distribution of the destruction rate, which is shown in Table 2, which shows, the emitted qi could kill the Hale cells. The destruction rate could reach 8.91, 59.61%, and the average destruction rate was 30.72 % (P<O.001).
At the same time, we observed the Hale cells with a translation electronmicroscope, and found that degeneration and swelling took place in some of the cells which received the emitted qi. The endoplasmic reticula were dilated obviously, and the nuclei of the cells were destroyed.
Table 2 Analysis of the destruction rate of the Hale cells by emitted qi
Destruction
rate (%) <10 11 21 31 41 >51 total
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Frequency 1 3 9 6 0 1 20
Ratio(%) 5 15 45 30 0 5 100
2. The effect of the emitted qi on the gastric adenocarcinoma cells:
After the gastric adenocarcinoma cells received the emitted qi for 1 hour, the survival rate of the cells was 74.98% of that in the control, i. e., the average destruction rate of the cells was 25.02%. (as shown in Table 3).
Table 3 The effect of the emitted qi on the gastric adenocarcinoma cells
Number of cells Survival Destruction
Group (1000/ml) rate rate
---- -------------- -------- -------
Exp. 51.12 100.00 0.O
Control 38.33 74.98 25.02
We repeated the experiment 11 times under identical conditions, and through statistical analysis there was a difference between the two groups (P<O.01 ). In the meantime, a scanning electronmicroscope was used to study the cells. The result showed that the microvilli of the cells which received the emitted qi had remarkably dwindled or disappeared and tiny holes could be seen on the surface of the cells.
3. The effect of the emitted qi on the chromosomes of the gastric adenocarcinoma cells:
We repeated the experiment 10 times under identical conditions. 1420 tumor cells were observed in the control group, and 1428 tumor cells were analyzed which received the emitted qi for 60 minutes. (See Table 4)
(l) The number of the chromosomes of the gastric adenocarcinoma cells: As Table 4 shows, the subdiploid in the control group was 2.11% . the supertriploid was 0.14%, whereas the subdiploid in the experiment group was 3.29%, and the supertriploid was 0.56%. There was a statistical difference in both conditions between the two groups (P<0.05). The abnormality rate of triploid and quadriploid in the experiment group and the control group had no statistical meaning (P>O.05)
(2)The structure of the chromosomes of the gastric adenocarcinoma cell: In the 1428 cells, the total abnormality rate was 5.39% in the experiment group, and the total abnormality rate of the 1420 cells in the control group was 1.40%, in which there was a statistical meaning (P < 0.01). The abnormality of the chromosomes included exchange, breaking and dicentromere.
Table 4 The effect of emitted qi on the chromosomes of the gastric adenocarcinoma cells
Abnormality rate of Abnormality rate
Number number of of structure of
Group (%) chromosomes chromosomes
Control Number <2n <3n <4n >4n 20 1.40
% 30 1370 13 2
Exp. Number 2.11 96.48 1.27 0.14
% 47 1349 24 8 77 5.39
3.29 94.46 1.68 0.56
P <0.05 >0.01 >0.01 <0.05 <0.01
Summary:
Tumor is a common disease which endangers man's life and health. The study on the effect of the emitted qi on carcinoma cells, especially the study of the structure and the chromosomes of tumor cells, has not been reported in the world and in our country. The result showed that:
1. The average destruction rate of the Hale cells by the emitted Qi was 30.72%, the highest destruction rate reaching 59.61%. In order to confirm the effect of the emitted qi, we repeated the experiment 20 times under identical conditions. Through a statistical analysis, there was a difference between the experiment group and the control group (P<O.01). We found that degeneration and swelling of the Hale cells took place. The endoplasmic reticula were dilated obviously, and the nuclei of the cells were destroyed after the cells received the emitted qi.
2. In 11 experiments, the average destruction rate of the gastric adenocarcinoma cells was 25.02% after they received the emitted qi for 60 minutes. There was a statistical meaning (P<O.01). At the same time, we asked some other qigong masters to participate in the same experiment, and same result was obtained.
In order to further confirm the authenticity, we used a scanning electronmicroscope to observe the cells. The observed result showed that the villi had come off or become shorter, fewer and scattered. The structure of the surface of the cells was disordered. Some holes were seen on the surface of the cells, and a few of the cells were destroyed completely.
3. The human chromosome is a material base of human heredity and a carrier of the genetic material. The results of our studies showed that the abnormality of the chromosomes of the gastric adenocarcinoma cells took place significantly after they received the emitted qi. The rate of exchange, breaking and dicentromere in the structure of the chromosomes of the gastric adenocarcinoma cells increased. There was a statistical difference between the experiment group and the control group. The result suggested that the emitted qi could kill the chromosomes of the gastric adenocarcinoma cells.
As shown above the results confirmed that the emitted qi could kill or inhibit the gastric adenocarcinoma cells and the Hale cells, and the effect of the emitted qi on tumor cells could be increased in vitro through self-regulation of a patient. The study may start a new way to cure carcinoma.
