Pulsed electromagnetic fields regulate metabolic reprogramming and mitochondrial fission in endothelial cells for angiogenesis
Author: Chengyi Yang#1,2, Li Xu#1, Feng Liao3, Chunmei Liao1, Yunying Zhao1, Yijie Chen1, Qian Yu1, Bo Peng4, Huifang Liu5
Affiliation:
1 Department of Rehabilitation Medicine, Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, 610072, People's Republic of China.
2 School of Medicine, University of Electronic Science and Technology of China, Chengdu, 610072, People's Republic of China.
3 Department of Orthopaedics, Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, 610072, People's Republic of China.
4 Department of Rehabilitation Medicine, Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, 610072, People's Republic of China. lucky63@163.com.
5 Department of Rehabilitation Medicine, Sichuan Provincial People's Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, 610072, People's Republic of China. liuhuifang@med.uestc.edu.cn.
Conference/Journal: Sci Rep
Date published: 2024 Aug 16
Other:
Volume ID: 14 , Issue ID: 1 , Pages: 19027 , Special Notes: doi: 10.1038/s41598-024-69862-x. , Word Count: 216
Pulsed electromagnetic field (PEMF) therapy has been extensively investigated in clinical studies for the treatment of angiogenesis-related diseases. However, there is a lack of research on the impact of PEMFs on energy metabolism and mitochondrial dynamics during angiogenesis. The present study included tube formation and CCK-8 assays. A Seahorse assay was conducted to analyze energy metabolism, and mitochondrial membrane potential assays, mitochondrial imaging, and reactive oxygen species assays were used to measure changes in mitochondrial structure and function in human umbilical vein endothelial cells (HUVECs) exposed to PEMFs. Real-time polymerase chain reaction was used to analyze the mRNA expression levels of antioxidants, glycolytic pathway-related genes, and genes associated with mitochondrial fission and fusion. The tube formation assay demonstrated a significantly greater tube network in the PEMF group compared to the control group. The glycolysis and mitochondrial stress tests revealed that PEMFs promoted a shift in the energy metabolism pattern of HUVECs from oxidative phosphorylation to aerobic glycolysis. Mitochondrial imaging revealed a wire-like mitochondrial morphology in the control group, and treatment with PEMFs led to shorter and more granular mitochondria. Our major findings indicate that exposure to PEMFs accelerates angiogenesis in HUVECs, likely by inducing energy metabolism reprogramming and mitochondrial fission.
Keywords: Angiogenesis; Energy metabolism; Mitochondria; Pulsed electromagnetic field.
PMID: 39152229 PMCID: PMC11329790 DOI: 10.1038/s41598-024-69862-x
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