Electromagnetic Fields Generated by the IteraCoil Device Differentiate Mesenchymal Stem Progenitor Cells Into the Osteogenic Lineage
Author: Gagik Greg Haroutunian1, Ashot Tsaghikian2, Elena Fedorova3, Pratima Chaurasia4, Gabriele Luca Gusella3, Arevik Mosoian3
Affiliation:
1 Keck School of Medicine, USC, Children's Hospital Los Angeles, Los Angeles, California.
2 Data Processing and Field Engineering Corp., Glendale, California.
3 SkinAxis LLC, Cedar Knolls, New Jersey.
4 Icahn School of Medicine at Mount Sinai, New York, New York.
Conference/Journal: Bioelectromagnetics
Date published: 2022 Apr 7
Other:
Special Notes: doi: 10.1002/bem.22401. , Word Count: 272
Rapid advances in mesenchymal stem progenitor cells (MSPCs) have rendered impetus into the area of cell therapy and regenerative medicine. The main promise of future stem cell therapies is their reliance on autologous stem cells derived from adipose tissue, which also includes treatments of bone fractures and degeneration. The effectiveness of different electric devices utilized to reprogram MSPCs toward osteogenic differentiation has provided varying degrees of effectiveness for clinical use. Adipose tissue-derived MSPCs were flow-cytometrically characterized and further differentiated into osteoblasts by culturing either in growth medium with pro-osteogenic supplements or without supplements with alternating electromagnetic field (EMF) generated by IteraCoil. IteraCoil is a multi-solenoid coil with a specific complex geometry that creates a 3D-EMF with desired parameters without directly applying electrodes to the cells and tissues. The flow-cytometric analysis of highly enriched (≥95%) adipose-derived MSPCs (CD34- , CD73+ , CD90+ , and CD105+ ) was utilized for the study. Osteoblasts and chondrocyte differentiations were then assessed by specific staining and quantified using ImageJ (National Institutes of Health). The osteoblastic differentiation of MSPCs cultured in regular medium and exposed to EMF at 0.05 and 1 kHz frequencies was compared with MSPCs cultured in a pro-osteogenic supplemented medium. In this study, we demonstrated that EMF from IteraCoil might have affected the signaling pathways that induce the osteogenic differentiation of human adipose-derived MSPCs in the absence of exogenous osteogenic factors. Therefore, EMF-generated osteogenic differentiation of reprogrammed adipose-derived autologous MSPCs may treat the loss of osteoblasts and osteoporosis and open new avenues for the development of regenerative cellular therapy. © 2022 Bioelectromagnetics Society.
Keywords: alternating current (AC); alternating electromagnetic field (EMF); chondrogenic differentiation; mesenchymal stem progenitor cells (MSPCs); osteogenic differentiation.
PMID: 35391494 DOI: 10.1002/bem.22401
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