Molecular biology study for the effect of qigong - outgoing qi with labeled biochemical factors on human body cancer cells the BEL - 7402 and SPC - A1

Author: Feng CY
Shanghai Academe of Chinese Traditional Medicine and Herbs Institute of Qigong, Shanghai, China
Conference/Journal: 7th Int Sym on Qigong
Date published: 1998
Other: Pages: 96 , Word Count: 477

Of recent years, owing to oncogene discovery, the start and regulation of growth by growth factors, and the appearance of many specific biochemical labeled factor detection methods makes for a deeper and better understanding of cancer disease. Chinese traditional medicine has been used over the years for the treatment of cancer. Qigong is one of the components of Chinese traditional medicine. Therefore, the study of biological effects of Qigong has Chinese characteristic subject.

In the past, we studied the effects of Qigong on the human liver cancer cell (BEL - 7402) and the lung adenoma cell (SPC - A13). It showed that Qigong had reversible and 'kill - wound' effects on these cancer cells. It this article, we carried out a determination and assay of cancer cell's specific biochemical labeled factors: ADH, ALDH, AFP, DNA, cell division cycle, nucleus type and phytoagglutinin reaction. These experiments pointed out that Qigong acted on BEL - 7402 cancer cells, ADH activity increased. ADH isoenzyme PAGF photogram indicated that, along with the increase of Qigong treatment numbers, fetes type ADH disappeared, but newborn type ADH appeared, and by inference ADH displayed reversibility.

After Qigong treatment on BEL - 7402 and SPC - Al cells, ALD activity decreased. ALD isoenzyme and PAGE photogram showed weakening of region B and more obvious weakening of region A. In other words, ALD was restored to normal tendency.

The experiment of Qigong acting on liver cancer cell Specific labeled factor - AFP showed that, along with the increase of Qigong treatment numbers, AFP content decreased step by step. It explained the comparatively forceful occurrence of cancer cell reversibility. After Qigong acted on cancer cell, ATP content in cancer cells increased. It was identical with the mitochondria morphological structure pertecely [present?] in cancer cell in which we published.

We used cancer cells in specific combinations with phytoagglutinin ConA to produce aggluitination for detecting the effect of Qigong on cell membrane function. Qigong decreased the degree of ConA - mediated cancer cell agglutination. We used PAGE photogran to analyze cell membrane glycoprotein. It showed the disappearance of high molecular weight glycoprotein bands, but increased some low molecular weight glycoprotein bands. It also illustrated that after Qigong acted on cancer cells, the decreased agglutination corresponded to transformation of protein molecules.

After Qigong acted on cancer cells, we analyzed cell division cycle and DNA content by FCM. We observed DNA high synthesis stage, its cell division frequency decreased, at the same time, the dyed DNA fluorescent intensity was also weakened. This indicated inhibition of DNA synthesis. It also was an index for cancer cell transformation to normal cells. Therefore, Qigong induced cancer cell reversibility, not only in the expression in cell structure and in the cell membrane composition and function, but also for hereditary, material structure and cell division cycle. All these experiments deepened our awareness to the cognition of cancer cell reversibility.