Author: Lu ZY1, Zhou HS2, Su ZX1,3, Qi J1,4, Zhang J4, Xue GH5, Li Y6, Hao CN5, Shi YQ7, Duan JL1
1Department of Gerontology, Xinhua Hospital, School of Medicine, Shanghai Jiaotong UniversityKongjiang Road 1665, Shanghai 200092, China.
2Shanghai Acoustics Laboratory, Chinese Academy of ScienceXiaomuqiao Road 456, Shanghai 200032, China.
3Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology130 Meilong Road, Shanghai 200237, China.
4Department of Pharmacy, Xinhua Hospital, School of Medicine, Shanghai Jiaotong UniversityKongjiang Road 1665, Shanghai 200092, China.
5Department of Vascular Surgery, Renji Hospital, School of Medicine, Shanghai Jiaotong UniversityDongfang Road 1630, Shanghai 200127, China.
6Division of Pulmonary and Critical Care Medicine, Department of Medicine, School of Medicine, University of Maryland20 Penn Street, HSF-2, Room #S112, Baltimore 21201, MD, USA.
7Department of Nephrology, Zhongshan Hospital, Fudan UniversityFenglin Road 180, Shanghai 200032, China.
Conference/Journal: Am J Transl Res.
Date published: 2017 Sep 15
Other: Volume ID: 9 , Issue ID: 9 , Pages: 4184-4194 , Word Count: 180
The objective of this investigation was to evaluate the therapy effect of combined therapeutic ultrasound (TUS) treatment and pulsed electromagnetic field (PEMF) therapy on angiogenesis in hypertension-related hindlimb ischemia. After subjecting excision of the left femoral artery, spontaneously hypertensive rats (SHRs) were randomly distributed to one of four groups: SHR; TUS treated SHR (SHR-TUS); PEMF treated SHR (PEMF-TUS); and TUS plus PEMF treated SHR (SHR-TUS-PEMF). Wistar-Kyoto rats (WKYs) with femoral artery excision were regarded as a control group. At day 14 after surgery, the TUS plus PEMF united administration had the greatest blood perfusion accompanied by elevated capillary density and the lowest TUNEL index. Interestingly, the united administration up-regulated the angiogenic factors expression of phosphorylated Akt (p-Akt), phosphorylated endothelial nitric oxide synthase (p-eNOS), vascular endothelial growth factor (VEGF), anti-apoptotic protein of Bcl-2 and down-regulated pro-apoptotic protein levels of Bax and Cleaved caspase-3 in vivo. Our results demonstrated that the united administration could significantly rescue hypertension-related inhibition of ischemia-induced neovascularization partly by promoting angiogenesis and inhibiting apoptosis.
KEYWORDS: Therapeutic ultrasound; peripheral arterial disease; pulsed electromagnetic field; spontaneously hypertensive rat
PMID: 28979692 PMCID: PMC5622261