miRNA expression profile is altered differentially in the rat brain compared to blood after experimental exposure to 50 Hz and 1 mT electromagnetic field.
Author: Erdal ME1, Yılmaz SG2, Gürgül S3, Uzun C4, Derici D5, Erdal N6
Affiliation:
1Faculty of Medicine, Department of Medical Biology and Genetics, Mersin University, Mersin 33343, Turkey. Electronic address: merdal@mersin.edu.tr.
2Faculty of Health Science, Department of Nutrition and Dietetics, Gaziantep University, Gaziantep, Turkey. Electronic address: gorucu@gantep.edu.tr.
3Faculty of Medicine, Department of Biophysics, Gaziosmanpaşa University, Tokat TR-60100, Turkey. Electronic address: sgurgul@gmail.com.
4Faculty of Medicine, Department of Biophysics, Mersin University, Mersin TR-33343, Turkey. Electronic address: cosaruzun@gmail.com.
5Faculty of Medicine, Department of Biostatistics and Medical Informatics, Mersin University, Mersin TR-33343, Turkey. Electronic address: didemderici@hotmail.com.
6Faculty of Medicine, Department of Biophysics, Mersin University, Mersin TR-33343, Turkey. Electronic address: nerdal@mersin.edu.tr.
Conference/Journal: Prog Biophys Mol Biol.
Date published: 2017 Aug 3
Other:
Pages: S0079-6107(17)30147-5 , Special Notes: doi: 10.1016/j.pbiomolbio.2017.08.001. [Epub ahead of print] , Word Count: 204
Common complex diseases are a result of host and environment interactions. One such putative environmental factor is the electromagnetic field exposure, especially the occupational extremely low frequency (ELF) magnetic field, 50 Hz, 1 mT, whose neurobiological relevance remains elusive. We evaluated the effects of long-term (60 days) ELF-MF exposure on miRNAs previously related to brain and human diseases (miR-26b-5p, miR-9-5p, miR-29a-3p, miR-106b-5p, miR-107, miR-125a-3p). A total of 64 young (3 weeks-old) and mature (10 weeks-old) male/female Wistar-Albino rats were divided into sham and ELF-MF exposed groups. After sacrifice of the animals, blood samples from rat's tail vein and brain tissues were collected. The expression levels of miRNAs were investigated with Real-Time PCR technique and TaqMan probe Technology. All miRNA expression levels of the young female rats show a significant decrease in blood according to brain samples (p < 0.05), but fewer miRNAs displayed a similar significant decrease in the blood. In conclusion, these new observations might inform future clinical biological psychiatry studies of long-term electromagnetic field exposure, and the ways in which host-environment interactions contribute to brain diseases.
Copyright © 2017 Elsevier Ltd. All rights reserved.
KEYWORDS: Alzheimer's disease; Blood; Brain; Electromagnetic field exposure; Host-environment interaction; MicroRNA; Rat
PMID: 28782562 DOI: 10.1016/j.pbiomolbio.2017.08.001
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