A Magnetically Actuated Microscaffold Containing Mesenchymal Stem Cells for Articular Cartilage Repair.

Author: Go G1,2, Han J1, Zhen J1,2, Zheng S1,2, Yoo A1, Jeon MJ1, Park JO1,2, Park S2,3
1Medical Microrobot Center (MRC), Robot Research Initiative (RRI), Chonnam National University, Gwangju, 500-480, South Korea.
2School of Mechanical Systems Engineering, Chonnam National University, Gwangju, 500-757, South Korea.
3Department of Robotics Engineering, Daegu Gyeongbuk Institute of Science and Technology, Daegu, 711-873, South Korea.
Conference/Journal: Adv Healthc Mater.
Date published: 2017 May 8
Other: Special Notes: doi: 10.1002/adhm.201601378. [Epub ahead of print] , Word Count: 228

This study proposes a magnetically actuated microscaffold with the capability of targeted mesenchymal stem cell (MSC) delivery for articular cartilage regeneration. The microscaffold, as a 3D porous microbead, is divided into body and surface portions according to its materials and fabrication methods. The microscaffold body, which consists of poly(lactic-co-glycolic acid) (PLGA), is formed through water-in-oil-in-water emulsion templating, and its surface is coated with amine functionalized magnetic nanoparticles (MNPs) via amino bond formation. The porous PLGA structure of the microscaffold can assist in cell adhesion and migration, and the MNPs on the microscaffold can make it possible to steer using an electromagnetic actuation system that provides external magnetic fields for the 3D locomotion of the microscaffold. As a fundamental test of the magnetic response of the microscaffold, it is characterized in terms of the magnetization curve, velocity, and 3D locomotion of a single microscaffold. In addition, its function with a cargo of MSCs for cartilage regeneration is demonstrated from the proliferation, viability, and chondrogenic differentiation of D1 mouse MSCs that are cultured on the microscaffold. For the feasibility tests for cartilage repair, 2D/3D targeting of multiple microscaffolds with the MSCs is performed to demonstrate targeted stem cell delivery using the microscaffolds and their swarm motion.

© 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

KEYWORDS: articular cartilage repair; magnetic field; mesenchymal stem cells; porous scaffold beads

PMID: 28481009 DOI: 10.1002/adhm.201601378