Effect of low-intensity pulsed ultrasound on osteogenic human mesenchymal stem cells commitment in a new bone scaffold.
Author: Carina V1,2, Costa V1,2, Raimondi L1,2, Pagani S3, Sartori M4, Figallo E5, Setti S6, Alessandro R7, Fini M3, Giavaresi G2,3
Affiliation:
1Rizzoli Orthopedic Institute, Bologna - Italy.
2Rizzoli Orthopedic Institute, Innovative Technological Platforms for Tissue Engineering, Theranostic and Oncology, Palermo - Italy.
3Rizzoli Orthopedic Institute, Laboratory of Preclinical and Surgical Studies, Bologna - Italy.
4Rizzoli Orthopedic Institute, Laboratory BITTA, Bologna - Italy.
5Fin-Ceramica Faenza SpA, Faenza (Ravenna) - Italy.
6IGEA SpA, Clinical Biophysics, Carpi (Modena) - Italy.
7Biology and Genetics Unit, Department of Biopathology and Medical Biotechnology, University of Palermo, Palermo - Italy.
Conference/Journal: J Appl Biomater Funct Mater.
Date published: 2017 Apr 28
Other:
Special Notes: doi: 10.5301/jabfm.5000342. [Epub ahead of print] , Word Count: 249
PURPOSE: Bone tissue engineering is helpful in finding alternatives to overcome surgery limitations. Bone growth and repair are under the control of biochemical and mechanical signals; therefore, in recent years several approaches to improve bone regeneration have been evaluated. Osteo-inductive biomaterials, stem cells, specific growth factors and biophysical stimuli are among those. The aim of the present study was to evaluate if low-intensity pulsed ultrasound stimulation (LIPUS) treatment would improve the colonization of an MgHA/Coll hybrid composite scaffold by human mesenchymal stem cells (hMSCs) and their osteogenic differentiation. LIPUS stimulation was applied to hMSCs cultured on MgHA/Coll hybrid composite scaffold in osteogenic medium, mimicking the microenvironment of a bone fracture.
METHODS: hMSCs were seeded on MgHA/Coll hybrid composite scaffold in an osteo-inductive medium and exposed to LIPUS treatment for 20 min/day for different experimental times (7 days, 14 days). The investigation was focused on (i) the improvement of hMSCs to colonize the MgHA/Coll hybrid composite scaffold by LIPUS, in terms of cell viability and ultrastructural analysis; (ii) the activation of MAPK/ERK, osteogenic (ALPL,COL1A1,BGLAP,SPP1) and angiogenetic (VEGF, IL8) pathways, through gene expression and protein release analysis, after LIPUS stimuli.
RESULTS: LIPUS exposure improved MgHA/Coll hybrid composite scaffold colonization and induced in vitro osteogenic differentiation of hMSCs seeded on the scaffold.
CONCLUSIONS: This work shows that the combined use of new biomimetic osteo-inductive composite and LIPUS treatment could be a useful therapeutic approach in order to accelerate bone regeneration pathways.
PMID: 28478615 DOI: 10.5301/jabfm.5000342
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