Osteogenic differentiation Potential of Mesenchymal Stem Cells cultured on Nanofibrous Scaffold Improved in the Presence of Pulsed Electromagnetic Field.
Author: Arjmand M1,2, Ardeshirylajimi A3, Maghsoudi H4, Azadian E2
Affiliation:
1Department of Biology, Payame Noor University (PNU), Tehran, Iran.
2Stem Cell Technology Research Center, Tehran, Iran.
3Department of Tissue engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
4Department of Biotechnology, Payame Noor University (PNU), Tehran, Iran.
Conference/Journal: J Cell Physiol.
Date published: 2017 Apr 17
Other:
Special Notes: doi: 10.1002/jcp.25962. [Epub ahead of print] , Word Count: 276
Nowadays, tissue engineering by using stem cells in combination with scaffolds and bioactive molecules has made significant contributions to the regeneration of damaged bone tissues. Since the usage of bioactive molecules including, growth factors to induce differentiation is safety limited in clinical applications, and it has also been previously observed that extremely low frequency pulsed electromagnetic fields (PEMF) can be effective in the enhancement of proliferation rate and osteogenic differentiation of stem cells, the aim of this study was investigating the osteoinductive potential of PEMF in combination with Poly(caprolactone) (PCL) nanofibrous scaffold. To achieve this aim, Adipose-derived mesenchymal stem cells (ADSCs) isolated and characterized and then osteogenic differentiation of them was investigated after culturing on the surface of PCL scaffold under treatments of PEMF, PEMF plus osteogenic medium (OM) and OM. Analysis of common osteogenic markers such as Alizarin red staining, ALP activity, calcium content and four important bone-related genes in days of 7, 14 and 21 confirmed that the effects of PEMF on the osteogenic differentiation of ADSCs are very similar to the effects of osteogenic medium. Thus, regarding the immunological concerns about the application of bioactive molecules for tissue engineering, PEMF could be a good alternative for osteogenic medium. Although, results were showed a synergetic effect for simultaneous application of PEMF and PCL scaffold in the osteogenesis process of ADSCs. Taking together, ADSCs-seeded PCL nanofibrous scaffold in combination with PEMF could be a great option for use in bone tissue engineering applications. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
KEYWORDS: Adipose-derived Mesenchymal Stem Cells; Electromagnetic Field; Extremely Low Frequency; Osteogenesis; Poly(caprolactone)
PMID: 28419435 DOI: 10.1002/jcp.25962
BACK
