Laser Acupuncture Exerts Neuroprotective Effects via Regulation of Creb, Bdnf, Bcl-2, and Bax Gene Expressions in the Hippocampus.

Author: Yun YC1, Jang D1, Yoon SB1, Kim D1, Choi DH1, Kwon OS2, Lee YM1, Youn D1
1Department of Meridian and Acupuncture Point, College of Korean Medicine, Dongshin University, 185, Geonjae-ro, Naju, Republic of Korea.
2Acupuncture, Moxibustion, and Acupuncture Point Research Group, Korea Institute of Oriental Medicine, 1672, Yuseong-daero, Yuseong-gu, Daejeon, Republic of Korea.
Conference/Journal: Evid Based Complement Alternat Med.
Date published: 2017
Other: Volume ID: 2017 , Pages: 7181637 , Special Notes: doi: 10.1155/2017/7181637. Epub 2017 Mar 20. , Word Count: 206

Acupuncture has a positive effect on cognitive deficits. However, the effects of laser acupuncture (LA) on cognitive function and its mechanisms of action are unclear. The present study aimed to evaluate the effects of LA on middle cerebral artery occlusion- (MCAO-) induced cognitive impairment and its mechanisms of action. Transient focal cerebral ischemia was modeled in adult Sprague-Dawley rats by MCAO. After LA or manual-acupuncture (MA) treatment at the GV20 and HT7 for 2 weeks, hippocampal-dependent memory was evaluated using the Morris water maze (MWM) test. The hippocampus was dissected to analyze choline acetyltransferase (ChAT) immunoreactivity and Creb, Bdnf, Bcl-2, and Bax gene expressions. MWM test demonstrated a significant improvement in hippocampal-dependent memory in the MCAO rats after LA treatment. LA treatment significantly reversed the postischemic decrease in ChAT immunoreactivity in the hippocampal CA1 region. LA treatment significantly normalized gene expression in the hippocampus which had been altered by MCAO, especially upregulating gene expression of Creb, Bdnf, and Bcl-2 and downregulating gene expression of Bax. This study suggests that LA treatment could improve cognitive impairment in MCAO rats to enhance the cholinergic system in the hippocampal CA1 region and to exert a neuroprotective effect by regulating Creb, Bdnf, Bcl-2, and Bax gene expressions.

PMID: 28408940 PMCID: PMC5376935 DOI: 10.1155/2017/7181637