Low-level infrared laser modulates muscle repair and chromosome stabilization genes in myoblasts.
Author: da Silva Neto Trajano LA1, Stumbo AC1, da Silva CL1, Mencalha AL2, Fonseca AS3,4
Affiliation:
1Laboratório de Pesquisa em Células Tronco, Departamento de Histologia e Embriologia, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Avenida 28 de Setembro, 87, fundos, Vila Isabel, Rio de Janeiro, 20551030, Brazil.
2Departamento de Biofísica e Biometria, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Avenida 28 de Setembro, 87, fundos, 4° andar, Vila Isabel, Rio de Janeiro, 20551030, Brazil.
3Departamento de Biofísica e Biometria, Instituto de Biologia Roberto Alcantara Gomes, Universidade do Estado do Rio de Janeiro, Avenida 28 de Setembro, 87, fundos, 4° andar, Vila Isabel, Rio de Janeiro, 20551030, Brazil. adnfonseca@yahoo.com.br.
4Departamento de Ciências Fisiológicas, Instituto Biomédico, Universidade Federal do Estado do Rio de Janeiro, Rua Frei Caneca, 94, Rio de Janeiro, 20211040, Brazil. adnfonseca@yahoo.com.br.
Conference/Journal: Lasers Med Sci.
Date published: 2016 May 25
Other:
Word Count: 205
Infrared laser therapy is used for skeletal muscle repair based on its biostimulative effect on satellite cells. However, shortening of telomere length limits regenerative potential in satellite cells, which occurs after each cell division cycle. Also, laser therapy could be more effective on non-physiologic tissues. This study evaluated low-level infrared laser exposure effects on mRNA expression from muscle injury repair and telomere stabilization genes in myoblasts in normal and stressful conditions. Laser fluences were those used in clinical protocols. C2C12 myoblast cultures were exposed to low-level infrared laser (10, 35, and 70 J/cm2) in standard or normal (10 %) and reduced (2 %) fetal bovine serum concentrations; total RNA was extracted for mRNA expression evaluation from muscle injury repair (MyoD and Pax7) and chromosome stabilization (TRF1 and TRF2) genes by real time quantitative polymerization chain reaction. Data show that low-level infrared laser increases the expression of MyoD and Pax7 in 10 J/cm2 fluence, TRF1 expression in all fluences, and TRF2 expression in 70 J/cm2 fluence in both 10 and 2 % fetal bovine serum. Low-level infrared laser increases mRNA expression from genes related to muscle repair and telomere stabilization in myoblasts in standard or normal and stressful conditions.
KEYWORDS: Low level laser; MyoD; Pax7; TRF1; TRF2
PMID: 27220530 [PubMed - as supplied by publisher]
BACK
