Extremely Low Frequency Electromagnetic Field in Mesenchymal Stem Cells Gene Regulation: Chondrogenic Markers Evaluation.
Author: Kavand H1,2, Haghighipour N1, Zeynali B3, Seyedjafari E4, Abdemami B1
Affiliation:
1National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran.
2Department of Cell and Molecular Biology, Tehran, Iran.
3Developmental Biology Lab, School of Biology, Tehran, Iran.
4Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran.
Conference/Journal: Artif Organs.
Date published: 2016 Apr 18
Other:
Special Notes: doi: 10.1111/aor.12696. [Epub ahead of print] , Word Count: 280
There is little evidence demonstrating the effects of electromagnetic fields (EMFs) generated within the biological entity and the effect of extrinsic fields on cellular programing. Taking the path of the more studied stimuli into attention, mechanical forces, it could be understood that nonchemical factors play a consequential role in transcriptional regulatory networks. Cartilaginous tissue consists of collagen protein that is considered as a piezoelectric substrate and is influenced by electric fields making chondrogenic specific genes an exciting candidate for bioelectromagnetic studies. As electromagnetic properties highly depend on the frequencies applied, this study delves into the ability of two EMFs with the frequency of 25 Hz and 50 Hz in inducing SOX9 and COL2 gene expressions in a three-dimensional (3D) mesenchymal stem cell (MSC)-alginate construct. Cell-alginate beads were divided into six groups and treated for a time period of 21 days. To determine the results, qualitative and quantitative data were both reviewed. On observation of real-time polymerase chain reaction (PCR) data, it was apparent that TGF-β1 treatment had a greater COL2 and SOX9 gene expression impact on MSCs compared to pulsed electromagnetic field (PEMF) treatments alone. COL2 was shown to have a greater transcriptional tendency to PEMF, whereas under defined electromagnetic parameters applied in this study, no significant difference was detected in SOX9 gene expressions compared to the control group. PEMF co-treatments enhanced the deposition of extracellular matrix molecules, as the matrix-rich beads were positively stained by Alcian blue. This genre of study is the venue for the control and healing of connective tissue defects.
Copyright © 2016 International Center for Artificial Organs and Transplantation and Wiley Periodicals, Inc.
KEYWORDS: Pulsed electromagnetic field-Mesenchymal stem cell-Cartilage tissue engineering-Electromagnetic transduction-Alginate
PMID: 27086585 [PubMed - as supplied by publisher]
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