Effect of pulsed electromagnetic field therapy on the osteogenic and adipogenic differentiation of bone marrow mesenchymal stem cells.
Author: Lu T1, Huang YX2, Zhang C1, Chai MX1, Zhang J1.
Affiliation:
1Second Department of Orthopedic Surgery, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, China. 2First Department of Orthopedic Surgery, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, China.
Conference/Journal: Genet Mol Res.
Date published: 2015 Sep 28
Other:
Volume ID: 14 , Issue ID: 3 , Pages: 11535-42 , Special Notes: doi: 10.4238/2015.September.28.5 , Word Count: 155
We investigated the effects of pulsed electromagnetic fields (PEMFs) of 20 Hz/2 mT on the osteogenic and adipogenic differentiation of bone marrow stem cells (BMSCs). Sprague Dawley rat BMSCs were isolated and cultured in vitro. The BMSCs of the third passage were obtained and stimulated by PEMFs of 20 Hz/2 mT. The alkaline phosphatase (ALP) activity was measured according to the ALP assay kit manufacturer instructions, the BMSC osteogenic and adipogenic indicators were detected by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR), and oil red O staining was used to observe the adipose-induced adipogenic differentiation of BMSCs. PEMFs of 20 Hz/2 mT significantly promoted the activity of ALP in the BMSCs (P < 0.01) and mRNA expression of osteogenic proteins (osteocalcin and osteopontin). The PEMFs inhibited the expression of adipogenic transcription factors such as adipokines and adipocyte-binding protein-2, and the adipogenic differentiation of BMSCs. PEMFs of 20 Hz/2 mT can promote osteogenic differentiation and inhibit adipogenic differentiation in BMSCs.
PMID: 26436394
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