Pulsed electromagnetic fields promote osteoblast mineralization and maturation needing the existence of primary cilia.
Author: Yan JL1, Zhou J1, Ma HP2, Ma XN1, Gao YH1, Shi WG1, Fang QQ1, Ren Q1, Xian CJ3, Chen KM4.
Affiliation:
1Institute of Orthopaedics,Lanzhou General Hospital, Lanzhou Command of CPLA, Lanzhou 730050, China. 2Department of Pharmacy, Lanzhou General Hospital, Lanzhou Command of CPLA, Lanzhou 730050, China. 3Sansom Institute for Health Research, School of Pharmacy and Medical Sciences, University of South Australia, Adelaide, SA 5001, Australia. 4Institute of Orthopaedics,Lanzhou General Hospital, Lanzhou Command of CPLA, Lanzhou 730050, China. Electronic address: chenkm@lut.cn.
Conference/Journal: Mol Cell Endocrinol.
Date published: 2015 Mar 15
Other:
Volume ID: 404 , Pages: 132-40 , Special Notes: doi: 10.1016/j.mce.2015.01.031 , Word Count: 165
Abstract
Although pulsed electromagnetic fields (PEMFs) have been approved as a therapy for osteoporosis, action mechanisms and optimal parameters are elusive. To determine the optimal intensity, exposure effects of 50 Hz PEMFs of 0.6-3.6 mT (0.6 interval at 90 min/day) were investigated on proliferation and osteogenic differentiation of cultured calvarial osteoblasts. All intensity groups stimulated proliferation significantly with the highest effect at 0.6 mT. The 0.6 mT group also obtained the optimal osteogenic effect as demonstrated by the highest ALP activity, ALP(+) CFU-f colony formation, nodule mineralization, and expression of COL-1 and BMP-2. To verify our hypothesis that the primary cilia are the cellular sensors for PEMFs, osteoblasts were also transfected with IFT88 siRNA or scrambled control, and osteogenesis-promoting effects of 0.6 mT PEMFs were found abrogated when primary cilia were inhibited by IFT88 siRNA. Thus primary cilia of osteoblasts play an indispensable role in mediating PEMF osteogenic effect in vitro.
Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.
KEYWORDS:
Differentiation; Mineralization; Osteoblasts; Primary cilia; Pulsed electromagnetic fields; RNA interference
PMID: 25661534
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