Author: An GZ1, Xu H2, Zhou Y1, Du L1, Miao X1, Jiang DP1, Li KC1, Guo GZ1, Zhang C3, Ding GR1.
Affiliation:
1Department of Radiation Medicine, Faculty of Preventive Medicine, The Fourth Military Medical University, Xi'an, Shaanxi province, China. 2Radiological College of Taishan Medical College, Taishan, Shandong province, China. 3China Academy of Telecommunication Research of Ministry of Industry and Information Technology, Beijing, China.
Conference/Journal: PLoS One.
Date published: 2015 Feb 19
Other:
Volume ID: 10 , Issue ID: 2 , Pages: e0117672 , Special Notes: doi: 10.1371/journal.pone.0117672 , Word Count: 238
Power-line frequency electromagnetic field (PF-EMF) was reported as a human carcinogen by some epidemiological research, but the conclusion is lack of robust experiment evidence. To identify the effects of long-term PF-EMF exposure on cell behavior, Balb/c 3T3 cells in exponential growth phase were exposed or sham-exposed to 50 Hertz (Hz) PF-EMF at 2.3 mT for 2 hours (h) one day, 5 days every week. After 11 weeks exposure, cells were collected instantly. Cell morphology was observed under invert microscope and Giemsa staining, cell viability was detected by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay, cell cycle and apoptosis was examined by flow cytometry, the protein level of Proliferating Cell Nuclear Antigen (PCNA) and CyclinD1 was detected by western blot, cell transformation was examined by soft agar clone assay and plate clone forming test, and cell migration ability was observed by scratch adhesion test. It was found that after PF-EMF exposure, cell morphology, apoptosis, cell migration ability and cell transformation didn't change. However, compared with sham group, cell viability obviously decreased and cell cycle distribution also changed after 11 weeks PF-EMF exposure. Meanwhile, the protein level of PCNA and CyclinD1 significantly decreased after PF-EMF exposure. These data suggested that although long-term 50Hz PF-EMF exposure under this experimental condition had no effects on apoptosis, cell migration ability and cell transformation, it could affect cell proliferation and cell cycle by down-regulation the expression of PCNA and CyclinD1 protein.
PMID: 25695503 [PubMed - in process] PMCID: PMC4335008