Neural circuits. Labeling of active neural circuits in vivo with designed calcium integrators.
Author: Fosque BF1, Sun Y1, Dana H1, Yang CT1, Ohyama T1, Tadross MR1, Patel R1, Zlatic M1, Kim DS1, Ahrens MB1, Jayaraman V1, Looger LL1, Schreiter ER2.
Affiliation:
1Howard Hughes Medical Institute, Janelia Farm Research Campus, 19700 Helix Drive, Ashburn, VA 20147, USA. 2Howard Hughes Medical Institute, Janelia Farm Research Campus, 19700 Helix Drive, Ashburn, VA 20147, USA. schreitere@janelia.hhmi.org.
Conference/Journal: Science.
Date published: 2015 Feb 13
Other:
Volume ID: 347 , Issue ID: 6223 , Pages: 755-60 , Special Notes: doi: 10.1126/science.1260922 , Word Count: 151
The identification of active neurons and circuits in vivo is a fundamental challenge in understanding the neural basis of behavior. Genetically encoded calcium (Ca(2+)) indicators (GECIs) enable quantitative monitoring of cellular-resolution activity during behavior. However, such indicators require online monitoring within a limited field of view. Alternatively, post hoc staining of immediate early genes (IEGs) indicates highly active cells within the entire brain, albeit with poor temporal resolution. We designed a fluorescent sensor, CaMPARI, that combines the genetic targetability and quantitative link to neural activity of GECIs with the permanent, large-scale labeling of IEGs, allowing a temporally precise "activity snapshot" of a large tissue volume. CaMPARI undergoes efficient and irreversible green-to-red conversion only when elevated intracellular Ca(2+) and experimenter-controlled illumination coincide. We demonstrate the utility of CaMPARI in freely moving larvae of zebrafish and flies, and in head-fixed mice and adult flies.
Copyright © 2015, American Association for the Advancement of Science.
PMID: 25678659
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