Author: Manaka S1, Tanabe N2, Kariya T3, Naito M4, Takayama T5, Nagao M1, Liu D6, Ito K7, Maeno M8, Suzuki N9, Miyazaki M5.
Affiliation:
1Nihon University Graduate School of Dentistry, Tokyo, Japan. 2Department of Biochemistry, Nihon University School of Dentistry, Tokyo, Japan; Division of Functional Morphology, Dental Research Center, Nihon University School of Dentistry, Tokyo, Japan. Electronic address: tanabe.natsuko@nihon-u.ac.jp. 3Department of Orthodontics, Nihon University School of Dentistry, Tokyo, Japan. 4Division of Functional Morphology, Dental Research Center, Nihon University School of Dentistry, Tokyo, Japan; Department of Anatomy, Nihon University School of Dentistry, Tokyo, Japan. 5Department of Periodontology, Nihon University School of Dentistry, Tokyo, Japan; Division of Advanced Dental Treatment, Dental Research Center, Nihon University School of Dentistry, Tokyo, Japan. 6Department of Prosthodontics, School of Dentistry, Shandong University, Jinan, Shandong Province, China. 7Nihon University, Tokyo, Japan. 8Division of Functional Morphology, Dental Research Center, Nihon University School of Dentistry, Tokyo, Japan; Department of Oral Health Sciences, Nihon University School of Dentistry, Tokyo, Japan. 9Department of Biochemistry, Nihon University School of Dentistry, Tokyo, Japan; Division of Functional Morphology, Dental Research Center, Nihon University School of Dentistry, Tokyo, Japan.
Conference/Journal: FEBS Lett.
Date published: 2014 Dec 24
Other:
Pages: S0014-5793(14)00893-X , Special Notes: doi: 10.1016/j.febslet.2014.12.013 , Word Count: 122
Abstract
Low-intensity pulsed ultrasound (LIPUS) is used for bone healing in orthopedics and dentistry. It has been shown that LIPUS induces the secretion of extracellular adenosine triphosphate (ATP), a key mediator of osteoblast response to mechanical stimuli. However, the detailed mechanism of LIPUS-induced osteogenesis has been elusive. In this study, we investigated the role of the P2X7 receptor in LIPUS-induced osteogenesis. LIPUS induced the release of extracellular ATP, differentiation of osteoblasts and osteogenesis via the P2X7 receptor, without affecting the activity of alkaline phosphatase (ALPase). These results suggest that LIPUS-induced extracellular ATP promotes bone formation via the osteoblast P2X7 receptor independently of ALPase.
Copyright © 2014. Published by Elsevier B.V.
KEYWORDS:
Adenosine triphosphate; Low-intensity pulsed ultrasound; Osteogenesis; P2X7
PMID: 25542352