Low-intensity pulsed ultrasound prompts tissue-engineered bone formation after implantation surgery.
Author: Wang J1, Wang J2, Yoshinori A3, Paul F4, Shen H5, Chen J3, Sotome S6, Liu Z1, Shinomiya K6.
Affiliation:
1Department of Orthopaedic Surgery, Xuanwu Hospital, Capital Medical University, Beijing 100053, China. 2Department of Orthopaedics, First Hospital of China Medical University, Shenyang, Liaoning 110001, China. 3Department of Orthopaedic Surgery, Graduate School, Tokyo Medical and Dental University, Tokyo 1138519, Japan. 4Department of Neurological Surgery, Wayne State University School of Medicine, Detroit, MI 48201, USA. 5Department of Orthopaedic Surgery, Xuanwu Hospital, Capital Medical University, Beijing 100053, China. Email: wangjywh@yahoo.com. 6Department of Orthopaedic Surgery, Graduate School, Tokyo Medical and Dental University, Tokyo 1138519, Japan; COE Program for Frontier Research on Molecular Destruction and Reconstruction of Tooth and Bone, Tokyo Medical and Dental University, Bunkyo-ku, Tokyo 1138519, Japan.
Conference/Journal: Chin Med J (Engl).
Date published: 2014 Feb
Other:
Volume ID: 127 , Issue ID: 4 , Pages: 669-74 , Word Count: 216
Abstract
BACKGROUND:
A practical problem impeding clinical translation is the limited bone formation seen in artificial bone grafts. Low-pressure/vacuum seeding and dynamic culturing in bioreactors have led to a greater penetration into the scaffolds, enhanced production of bone marrow cells, and improved tissue-engineered bone formation. The goal of this study was to promote more extensive bone formation in the composites of porous ceramics and bone marrow stromal cells (BMSCs).
METHODS:
BMSCs/β-tricalcium phosphate (β-TCP) composites were subcultured for 2 weeks and then subcutaneously implanted into syngeneic rats that were split into a low-intensity pulsed ultrasound (LIPUS) treatment group and a control group. These implants were harvested at 5, 10, 25, and 50 days after implantation. The samples were then biomechanically tested and analyzed for alkaline phosphate (ALP) activity and osteocalcin (OCN) content and were also observed by light microscopy.
RESULTS:
The levels of ALP activity and OCN content in the composites were significantly higher in the LIPUS group than in the control group. Histomorphometric analysis revealed a greater degree of soft tissue repair, increased blood flow, better angiogenesis, and more extensive bone formation in the LIPUS groups than in the controls. No significant difference in the compressive strength was found between the two groups.
CONCLUSION:
LIPUS treatment appears to enhance bone formation and angiogenesis in the BMSCs/β-TCP composites.
PMID: 24534220
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