Author: Fu N, Yang X, Ba K, Fu Y, Wei X, Yue Y, Li G, Yao Y, Chen J, Cai X, Liang C, Ge Y, Lin Y.
Affiliation: State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, 610041, China.
Conference/Journal: Cell Prolif.
Date published: 2013 Jun
Other:
Volume ID: 46 , Issue ID: 3 , Pages: 312-9 , Special Notes: doi: 10.1111/cpr.12031 , Word Count: 111
OBJECTIVE:
The aim of this study was to investigate effects of low-intensity pulsed ultrasound (LIPUS) on differentiation of adipose-derived stem cells (ASCs), in vitro.
MATERIALS AND METHODS:
Murine ASCs were treated with LIPUS for either three or five days, immediately after adipogenic induction, or delayed for 2 days. Expression of adipogenic genes PPAR-γ1, and APN, was examined by real-time PCR. Immunofluorescence (IF) staining was performed to test for PPAR-γ at the protein level.
RESULTS:
Our data revealed that specific patterns of LIPUS up-regulated levels of both PPAR-γ1 and APN mRNA, and PPAR-γ protein.
CONCLUSIONS:
In culture medium containing adipogenic reagents, LIPUS enhanced ASC adipogenesis.
© 2013 Blackwell Publishing Ltd.
PMID: 23692089 [PubMed - in process]