This paper is a primary study about the biological effect of the emitted qi of qigong on the basis of enzyme study. According to known reports, we know that the emitted qi of qigong can raise the budding rate of rice seeds. Our experiment also shows that supplying the emitted qi to wheat seeds and corn seeds for a period of time can affect them when they later begin budding. If the purpose of the qigong supplier is to promote the budding, the budding rate may be comparatively enhanced. The budding process of seeds is a very complex physiological and biochemical one. What actual segment the emitted qi affects is something still unknown.

Wheat seeds contain 72 percent starch, which is a fairly high amount. The starch hydrolyzes and becomes the main source of nourishing matter needed by the embryo's growth. The hydrolysis of starch calls for alpha-amylase(a-amylase) and beta-amylase(ß-amylase). ß-amylase exists even before the budding of the seeds, and it forms quickly when the seeds are swelling and it is formed from the zymogen which formerly existed in the endosperm. Usually it begins to show its vitality three hours after swelling. The production of a-amylase is more complex when the seeds bud, the embryo produces gibberellin (GA). GA is transported to the endosperm and promotes the composition of some particular mRNA, therefore translating a particular protein --a-amylase. It shows its vitality much later than ß-amylase, as it shows after the seeds are drenched for 21 hours. The inductivity of GA to a-amylase is a very specific reaction. If we deprive the seeds of their embryos, without the inductivity of GA the seeds cannot produce a-amylase. The exogenous GA has a similar inducing function.

Before the experiment we cut off the embryos of the wheat seeds and cultivated them for 28 hours. After supplying the emitted qi to the experimental group, the seeds were cultivated for another 21 hours. Then the amylase vitality of the seeds was tested and compared with that of the control group. The result is that the difference between them is not obvious (P> 0.05). The experiment was repeated.

In addition to the qigong group and the control group, we had two other groups the group of seeds supplied with GA, the group of seeds supplied with the emitted qi and GA. After using the emitted qi for 40 hours, the vitality of each group was tested respectively, and the results were compared. We found that the vitality of the group supplied with the emitted qi was much higher than that of the control group (P< 0.05) and the emitted qi did not have much effect on the GA treated group (P<0.05). The experiment shows that the emitted qi can affect the vitality of wheat seeds. The influence shows very obvious after the supplying of the emitted qi for a comparatively long period (more than 24 hours). This matches with the fact that a-amylase shows its vitality after a long period. We can draw a primary conclusion the emitted qi of qigong mainly has an effect on the vitality of a-amylase. In the study of the inducing function of GA on the biological composition of a-amylase it is believed that GA takes part in the translating process of protein. The emitted qi has the same inducing function as GA. Its mechanism might also have something to do with this. Besides, when GA exists, the inducing function of the emitted qi is not obvious, which also shows that they may compete with each other, just because they have a similar affecting point in the composition of a-amylase.

This experiment is only a primary study of the microcosmic mechanism of the biological effect of qigong. Its conclusion needs to be supported by other scientific experiments.

Study of the inducing function of the emitted qi of qigong on the biological composition of a-amylase in wheat seeds