Histological aspect of threadlike structure inside blood vessel

P.473

Histological aspect of threadlike structure inside blood vessel

Baik Ku-Yooun 1//Park Eun-Sung 2//Lee Byung-Cheon 1//Shin Hak-Soo 1////


Biomedical Physics Lab., School of Physics, Seoul National University, Seoul, 151-747, Korea [1]//Department of Anatomy and Cell Biology, College of Veterinary Medicine, Seoul National University [2]

We report on the observation of a novel structure inside of the blood vessels in rats. This is a rediscovery of the Bonghan ducts, which have not been yet confirmed because the observing method was not known. We applied a new procedure for observing these intra-blood vessel structures. The method we contrived is to let blood be coagulated around them so that they become thick and strong by intravenous injection of dextrose solution into a femoral vein. A piece of thickened sample is treated with urokinase to remove fibrin and blood clots and to recover the thin thread. Histological study was done with hematoxylin and eosin, Mallory's triple and Verhoeff's elastic stains. This intra-vascular thread was a semi-transparent and elastic structure whose diameters was of about 20 100 µm and also had many myoid cell-like rod-shape nuclei.
Keywords: Intra-vascular thread, Bonghan duct, Hematoxylin and Eosin, Mallory's triple and Verhoeff's elastic stain