Author: Escobar JF1,2, Vaca-González JJ1,3, Guevara JM4, Vega JF5, Hata YA1,2, Garzón-Alvarado DA1,2
Affiliation: <sup>1</sup>Biomimetics Laboratory, Instituto de Biotecnología, Universidad Nacional de Colombia, Bogotá, Colombia.
<sup>2</sup>Numerical Methods and Modeling Research Group (GNUM), Universidad Nacional de Colombia, Bogotá, Colombia.
<sup>3</sup>Nefertiti, Wellness and New Technologies, Surgical Instrumentation Department, Fundación Universitaria del Área Andina, Bogotá, Colombia.
<sup>4</sup>Institute for the Study of Inborn Errors of Metabolism, Pontificia Universidad Javeriana, Bogotá, Colombia.
<sup>5</sup>Electromagnetic Compatibility Research Group (EMC-UN), Universidad Nacional de Colombia, Bogotá, Colombia.
Conference/Journal: Bioelectromagnetics.
Date published: 2019 Nov 17
Other:
Special Notes: doi: 10.1002/bem.22231. [Epub ahead of print] , Word Count: 211
Magnetic fields (MFs) have been used as an external stimulus to increase cell proliferation in chondrocytes and extracellular matrix (ECM) synthesis of articular cartilage. However, previously published studies have not shown that MFs are homogeneous through cell culture systems. In addition, variables such as stimulation times and MF intensities have not been standardized to obtain the best cellular proliferative rate or an increase in molecular synthesis of ECM. In this work, a stimulation device, which produces homogeneous MFs to stimulate cell culture surfaces was designed and manufactured using a computational model. Furthermore, an in vitro culture of primary rat chondrocytes was established and stimulated with two MF schemes to measure both proliferation and ECM synthesis. The best proliferation rate was obtained with an MF of 2 mT applied for 3 h, every 6 h for 8 days. In addition, the increase in the synthesis of glycosaminoglycans was statistically significant when cells were stimulated with an MF of 2 mT applied for 5 h, every 6 h for 8 days. These findings suggest that a stimulation with MFs is a promising tool that could be used to improve in vitro treatments such as autologous chondrocyte implantation, either to increase cell proliferation or stimulate molecular synthesis. Bioelectromagnetics.
© 2019 Bioelectromagnetics Society.
KEYWORDS: articular cartilage; cell proliferation; chondrocyte; glycosaminoglycans; magnetic fields
PMID: 31736106 DOI: 10.1002/bem.22231