Many studies of the physical and biological effects and the clinical practice with the emitted qi have been reported. The aim of the study in this paper is to look into the material basis and the mechanism of the emitted qi by chemical methods. It includes three aspects (l) Thermal efficiency of the emitted qi on various chemicals; (2) Effect of the emitted qi on the biological activity of enzymes; (3) Effect of the emitted qi on the conformation of proteins.

1. Thermal efficiency of the emitted qi on various chemicals.
Acetone, ether and absolute alcohol were poured into three 25 ml. flasks respectively, and then were acted upon by the emitted qi from three qigong masters, A, B and D. After a few minutes, it was found that the temperature of each chemical rose to some extent. With statistic evaluating, we found a linear relation of the logarithm of the change in the calorific value Q of each chemical to the distance S from the flask bottom to the qigong master's point Laogong (P 8). The linear relation appeared in nine experiments out of twelve.

It is very interesting that for the three chemicals, each slops of this kind of plot, i. e., lgQ vs. S, keeps a constant, if the source of the emitted qi is from the same qigong master. Therefore, an equation to compare the two values Q and S separately is got through a comparison between the qigong master and non- qigong master.

Q = e0.434(bs+a)

Where 'a' is a constant relating to the thermal efficiency of the chemicals on which the emitted qi acts; 'b' is an attenuation coefficient reflecting the decrease in IgQ with the increase in S. And, the larger the b value, the slower the attenuation rate is.
It was also found that the emitted qi is not a thermal current, it is a form of energy, which can transmit in air and through glass. The thermal efficiency is only produced by the interactions of the emitted qi and the receivers.

2. Effect of the emitted qi on the enzymatic activity.
The activity of saccharogenic amylase was proved by receiving twelve times of the emitted qi of the three qigong masters, B, C, c. The result was got by statistic method. The activity of saccharogenic amylase was decreased when they were acted on by B and C, and the maximum value was -20% . But when D acted on it for three times. the activity of saccharogenic amylase increased and for once it decreased. The maximum was 10%. When D acted on a-amylase, the activity of it changed too. Substrates, pH values, temperature, allosteric effect, activator. inhibitor, etc. were the influential factors on the activity of enzyme. Since all the experimental conditions were controlled strictly, the only cause for the change in the activities of enzymes in the study comes from the change in the allosteric effect of each enzyme.

3. Influence of the emitted qi on the conformation of proteins.
To prove the possibility that the activity of protein may be changed by the emitted qi, we designed experiments of high performance liquid chromatography (HPLC) . Rigid cation exchanger was chosen as the stationary phase and many salt aqueous solutions with a suitable pH value were chosen as the mobile phases. Results showed the decrease in tr (retention time) of Iysozyme, when the emitted qi acted on the column, or the column oven, or the sample solution in a tube directly. The decreased range of its activity was over -3.5% to -17.5%. The fact elucidates that the emitted qi has an ability to pass through the wall of the stainless column, then it interacts with protein and makes the change in its conformation. When cytochrome C received the emitted qi by the same qigong master, time became obviously longer. The corresponding increase in Z value, Delta Z, was 1.49. The phenomenon explained the increases in the total number of the cation and the anion released from the contact interface between cytochrome C molecule and the surface of the stationary phase. The contact surface was enlarged to an extent that changed its structure when it participated in the chromatographic process.

Study of the mechanism of emitted qi