Author: Shi J1, Pang L2, Jiao S3
Affiliation: <sup>1</sup>Liaocheng People's Hospital and Liaocheng Clinical School of Taishan Medical University, Liaocheng, China.
<sup>2</sup>Department of Spine Surgery, Liaocheng People's Hospital, Liaocheng, 252000, Shandong, China, Liaocheng, China.
<sup>3</sup>Department of Orthopaedics, Yantai Yeda Hospital, Yantai, 264006, Shandong, China, Liaocheng, China shouguojiao0113@sina.com.
Conference/Journal: Biosci Rep.
Date published: 2018 Feb 5
Other:
Pages: BSR20180064 , Special Notes: doi: 10.1042/BSR20180064. [Epub ahead of print] , Word Count: 185
BACKGROUND: Mechanical stimuli obviously affect disc nucleus pulposus (NP) biology. Previous studies have indicate that static compression exhibits detrimental effects on disc biology compared with dynamic compression.
OBJECTIVE: To study disc NP cell senescence under static compression and dynamic compression in a disc organ culture.
METHODS: Porcine discs were cultured and subjected to compression (static compression: 0.4 MPa for 4 hours once per day; dynamic compression: 0.4 MPa at a frequency of 1.0 Hz for 4 hours once per day) for 7 days using a self-developed mechanically active bioreactor. The non-compressed discs were used as controls.
RESULTS: Compared with the dynamic compression, static compression significantly promoted disc NP cell senescence, reflected by the increased senescence associated β-galactosidase (SA-β-Gal) activity, senescence-associated heterochromatic foci (SAHF) formation and senescence markers expression, and the decreased telomerase activity and NP matrix biosynthesis.
CONCLUSION: Static compression accelerates disc NP cell senescence compared with the dynamic compression in a disc organ culture. This study provides that acceleration of NP cell senescence may be involved in previously reported static compression-mediated disc NP degenerative changes.
©2018 The Author(s).
KEYWORDS: compression; degeneration; nucleus pulposus; organ culture; senescence
PMID: 29437905 DOI: 10.1042/BSR20180064