Author: Bayat M1, Virdi A2, Rezaei F3, Chien S4
Affiliation: <sup>1</sup>Price Institute of Surgical Research, University of Louisville, Louisville, KY, USA; Cellular and Molecular Biology Research Center, Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Electronic address: bayat_m@yahoo.com.
<sup>2</sup>Department of Cell & Molecular Medicine, (Formerly, Anatomy and Cell Biology), Rush University Medical Center, 1750 W. Harrison St., Suite 1413A, Chicago Il 60612, USA. Electronic address: amarjit_virdi@rush.edu.
<sup>3</sup>Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Electronic address: journalist.best@gmail.com.
<sup>4</sup>Noveratech LLC of Louisville, KY, USA; Price Institute of Surgical Research, Hiram C Polk Jr. MD Department of Surgery, University of Louisville, Louisville, KY, USA. Electronic address: sufanc@aim.com.
Conference/Journal: Prog Biophys Mol Biol.
Date published: 2017 Nov 7
Other:
Pages: S0079-6107(17)30208-0 , Special Notes: doi: 10.1016/j.pbiomolbio.2017.11.001. [Epub ahead of print] , Word Count: 315
To compare the in vitro effectiveness of Low-Level Laser Therapy (LLLT) and Low Intensity Pulsed Ultrasound (LIPUS) on bony cells and related stem cells. In this study, we aim to systematically review the published scientific literature which explores the use of LLLT and LIPUS to biostimulate the activity or the proliferation of bony cells or stem cells in vitro. We searched the database PubMed for LLLT or LIPUS, with/without bone, osteoblast, osteocyte, stem cells, the human osteosarcoma cell line (MG63), bone-forming cells, and cell culture (or in vitro). These studies were subdivided into categories exploring the effect of LLLT or LIPUS on bony cells, stem cells, and other related cells. 75 articles were found between 1987 and 2016; these included: 50 full paper articles on LLLT and 25 full papers on LIPUS. These articles met the eligibility criteria and were included in our review. A detailed and concise description of the LLLT and the LIPUS protocols and their individual effects on bony cells or stem cells and their results are presented in five tables. Based on the main results and the conclusions of the reviewed articles in the current work, both, LLLT and LIPUS, apply a biostimulatory effect on osteoblasts, osteocytes, and enhance osteoblast proliferation and differentiation on different bony cell lines used in in vitro studies, and therefore, these may be useful tools for bone regeneration therapy. Moreover, in consideration of future cell therapy protocols, both, LLLT and LIPUS (especially LLLT), enhnce a significant increase in the initial number of SCs before differentiation, thus increasing the number of differentiated cells for tissue engineering, regenerative medicine, and healing. Further studies are necessary to determine the LLLT or the LIPUS parameters, which are optimal for biostimsulating bony cells and SCs for bone healing and regenerative medicine.
Copyright © 2017. Published by Elsevier Ltd.
KEYWORDS: In vitro; Low intensity pulsed ultrasound; Low-level laser therapy; Osteoblast; Osteocyte; Photobiomodulation; Stem cells
PMID: 29126668 DOI: 10.1016/j.pbiomolbio.2017.11.001