Author: Sepehrimanesh M1,2, Kazemipour N3, Saeb M3, Nazifi S4, Davis DL5
Affiliation: <sup>1</sup>Gastroenterohepatology Research Center, Shiraz University of Medical Sciences, Po. Box: 17935-1311, Shiraz, Iran. sepehrimaneshmasood@gmail.com.
<sup>2</sup>Department of Biochemistry, School of Veterinary Medicine, Shiraz University, Shiraz, Iran. sepehrimaneshmasood@gmail.com.
<sup>3</sup>Department of Biochemistry, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
<sup>4</sup>Department of Clinical Pathology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.
<sup>5</sup>Environmental Health Trust, Teton Village, WY, USA.
Conference/Journal: Environ Sci Pollut Res Int.
Date published: 2017 Apr 10
Other:
Special Notes: doi: 10.1007/s11356-017-8882-z. [Epub ahead of print] , Word Count: 216
Although cell phones have been used worldwide, some adverse and toxic effects were reported for this communication technology apparatus. To analyze in vivo effects of exposure to radiofrequency-electromagnetic field (RF-EMF) on protein expression in rat testicular proteome, 20 Sprague-Dawley rats were exposed to 900 MHz RF-EMF for 0, 1, 2, or 4 h/day for 30 consecutive days. Protein content of rat testes was separated by high-resolution two-dimensional electrophoresis using immobilized pH gradient (pI 4-7, 7 cm) and 12% acrylamide and identified by MALDI-TOF/TOF-MS. Two protein spots were found differentially overexpressed (P < 0.05) in intensity and volume with induction factors 1.7 times greater after RF-EMF exposure. After 4 h of daily exposure for 30 consecutive days, ATP synthase beta subunit (ASBS) and hypoxia up-regulated protein 1 precursor (HYOU1) were found to be significantly up-regulated. These proteins affect signaling pathways in rat testes and spermatogenesis and play a critical role in protein folding and secretion in the endoplasmic reticulum. Our results indicate that exposure to RF-EMF produces increases in testicular proteins in adults that are related to carcinogenic risk and reproductive damage. In light of the widespread practice of men carrying phones in their pockets near their gonads, where exposures can exceed as-tested guidelines, further study of these effects should be a high priority.
KEYWORDS: Electromagnetic wave; Heat shock proteins; Proteome; Two-dimensional electrophoresis
PMID: 28397118 DOI: 10.1007/s11356-017-8882-z