Author: Uddin SM1, Richbourgh B2, Ding Y2, Hettinghouse A2, Komatsu DE1, Qin YX3, Liu CJ4
Affiliation: <sup>1</sup>Department of Orthopaedics, Stony Brook University, Stony Brook, NY 11794, USA.
<sup>2</sup>Department of Orthopedic Surgery, Hospital of Joint Disease, New York University Medical Center, New York, NY 10003, USA.
<sup>3</sup>Department of Biomedical Engineering, Stony Brook University, Stony Brook, NY 11794, USA.
<sup>4</sup>Department of Orthopedic Surgery, Hospital of Joint Disease, New York University Medical Center, New York, NY 10003, USA. Electronic address: Chuanju.liu@med.nyu.edu.
Conference/Journal: Osteoarthritis Cartilage.
Date published: 2016 Nov
Other:
Volume ID: 24 , Issue ID: 11 , Pages: 1989-1998 , Special Notes: doi: 10.1016/j.joca.2016.06.014. Epub 2016 Jun 27. , Word Count: 248
OBJECTIVES: Cartilage is a highly mechano-responsive tissue. Chondrocytes undergo a series of complex changes, including proliferation and metabolic alteration as the target of external biomechanical and biochemical stimuli. IL-1β is known to regulate chondrocyte metabolism and plays an important role in the pathogenesis of osteoarthritis (OA). The objective of this study was to employ low-intensity pulsed ultrasound (LIPUS) as a localized mechanical stimulus and assess its effects on chondrocyte migration, proliferation, metabolism, and differentiation, as well as its ability to suppress IL-1β mediated catabolism in cartilage.
METHODS: Human cartilage explants and chondrocytes were stimulated by LIPUS in the presence and absence of IL-1β to asses cartilage degradation, chondrocytes metabolism, migration, and proliferation. Western blot analyses were conducted to study IL-1β the associated NFκB pathway in chondrocytes.
RESULTS: LIPUS stimulation increased the proteoglycan content in human cartilage explants and inhibited IL-1β induced loss of proteoglycans. LIPUS stimulation increased rates of chondrocyte migration and proliferation, and promoted chondrogenesis in mesenchymal stem cells (MSC). Further, LIPUS suppressed IL-1β induced activation of phosphorylation of NFκB-p65 and IĸBα leading to reduced expression of MMP13 and ADAMT5 in chondrocytes.
CONCLUSIONS: Collectively, these data demonstrate the potential therapeutic effects of LIPUS in preventing cartilage degradation and treating OA via a mechanical stimulation that inhibits the catabolic action of IL-1β and stimulates chondrocyte migration, proliferation, and differentiation.
Copyright © 2016 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.
KEYWORDS: Cartilage metabolism; Chondrocytes; Interleukin 1β; Low intensity pulsed ultrasound; Osteoarthritis
PMID: 27364595 DOI: 10.1016/j.joca.2016.06.014