Author: Xu H1, Zhang J1, Lei Y1, Han Z1, Rong D1, Yu Q1, Zhao M2, Tian J3
Affiliation: <sup>1</sup>Department of Orthopaedics, Zhujiang Hospital, Southern Medical University, 253 Industrial Avenue, Haizhu, Guangzhou 510280, China.
<sup>2</sup>Department of Pathophysiology, Basic Medical College, Southern Medical University, Baiyun, Guangzhou 510515, China.
<sup>3</sup>Department of Orthopaedics, Zhujiang Hospital, Southern Medical University, 253 Industrial Avenue, Haizhu, Guangzhou 510280, China. Electronic address: tianjing_ortho@163.com.
Conference/Journal: Biochem Biophys Res Commun.
Date published: 2016 Sep 11
Other:
Pages: S0006-291X(16)31495-4 , Special Notes: doi: 10.1016/j.bbrc.2016.09.044. [Epub ahead of print] , Word Count: 197
Low frequency pulsed electromagnetic field (PEMF) has been shown to affect the activity of various cell types and promote them proliferation. However, its effect on skeletal muscle cells remains to be determined. In our study, we confirmed that PEMF (100 Hz, 1 mT) could promote C2C12 myoblasts proliferation by using Cell Counting Kit-8 (CCK-8) and 5-Ethynyl-2'-deoxyuridine (EdU) assays, yet hardly any distinction was found in the rate of cell apoptosis between PEMF and control groups by flow cytometry (Annexin V-FITC/PI double staining method). To further study the mechanism of action of PEMF, Western blot was utilized to detect the mitogen-activated protein kinase (MAPK) signaling pathways. After exposing C2C12 myoblasts to PEMF, we found the phosphorylation level of extracellular signal-regulated kinase (ERK) was significantly increased, while p38 MAPK and c-Jun N-terminal kinase (JNK) pathways were not affected. Pretreating the cells with the ERK kinase1/2 (MEK1/2) inhibitor U0126 obviously inhibited the proliferation of C2C12 cells. Taken together, our research for the first time demonstrated that PEMF promoted C2C12 myoblasts proliferation via activating MAPK/ERK pathway.
Copyright © 2016. Published by Elsevier Inc.
KEYWORDS: C2C12 myoblasts; MAPK/ERK pathway; Proliferation; Pulsed electromagnetic field
PMID: 27629357 DOI: 10.1016/j.bbrc.2016.09.044