Author: Huser T1, Chan J2
Affiliation: <sup>1</sup>Department of Physics, University of Bielefeld, Universitätsstr. 25, 33615 Bielefeld, Germany; Department of Internal Medicine, University of California, Davis, Sacramento, CA 95817, United States; NSF Center for Biophotonics Science and Technology, University of California, Davis, 2700 Stockton Blvd., Suite 1400, Sacramento, CA 95817, United States. Electronic address: thomas.huser@physik.uni-bielefeld.de.
<sup>2</sup>NSF Center for Biophotonics Science and Technology, University of California, Davis, 2700 Stockton Blvd., Suite 1400, Sacramento, CA 95817, United States; Department of Pathology and Laboratory Medicine, University of California, Davis, Sacramento, CA 95817, United States. Electronic address: jwjchan@ucdavis.edu.
Conference/Journal: Adv Drug Deliv Rev.
Date published: 2015 Jul 15
Other:
Volume ID: 89 , Pages: 57-70 , Special Notes: doi: 10.1016/j.addr.2015.06.011. Epub 2015 Jul 3. , Word Count: 173
Raman micro-spectroscopy provides a convenient non-destructive and location-specific means of probing cellular physiology and tissue physiology at sub-micron length scales. By probing the vibrational signature of molecules and molecular groups, the distribution and metabolic products of small molecules that cannot be labeled with fluorescent dyes can be analyzed. This method works well for molecular concentrations in the micro-molar range and has been demonstrated as a valuable tool for monitoring drug-cell interactions. If the small molecule of interest does not contain groups that would allow for a discrimination against cytoplasmic background signals, "labeling" of the molecule by isotope substitution or by incorporating other unique small groups, e.g. alkynes provides a stable signal even for time-lapse imaging such compounds in living cells. In this review we highlight recent progress in assessing the physiology of cells and tissue by Raman spectroscopy and imaging.
Copyright © 2015 Elsevier B.V. All rights reserved.
KEYWORDS: Cell physiology; Coherent Raman scattering; Inelastic light scattering; Label-free optical microscopy; Raman scattering; Single cell analysis; Tissue physiology
PMID: 26144996 [PubMed - indexed for MEDLINE]