Author: Falone S1, Marchesi N2, Osera C2, Fassina L3,4, Comincini S5, Amadio M2, Pascale A2.
Affiliation: 1a Department of Life, Health and Environmental Sciences , University of L'Aquila , L'Aquila, Italy ; 2b Department of Drug Sciences , Section of Pharmacology, University of Pavia, Pavia, Italy ; 3c Department of Electrical , Computer and Biomedical Engineering, University of Pavia, Pavia, Italy ; 4d Centre for Health Technologies (C.H.T.), University of Pavia, Pavia, Italy ; 5e Department of Biology and Biotechnology , University of Pavia , Pavia , Italy.
Conference/Journal: Int J Radiat Biol.
Date published: 2016 Mar 4
Other:
Volume ID: 1-6 , Word Count: 192
Purpose The redox milieu, together with reactive oxygen species (ROS) accumulation, may play a role in mediating some biological effects of extremely-low-frequency electromagnetic fields (ELF-EMF). Some of us have recently reported that a pulsed EMF (PEMF) improves the antioxidant response of a drug-sensitive human neuroblastoma SH-SY5Y cell line to pro-oxidants. Since drug resistance may affect cell sensitivity to redox-based treatments, we wanted to verify whether drug-resistant human neuroblastoma SK-N-BE(2) cells respond to a PEMF in a similar fashion. Materials and methods SK-N-BE(2) cells were exposed to repeated 2 mT, 75 Hz PEMF (15 min each, repeated 3 times over 5 days), and ROS production, Mn-dependent superoxide dismutase (MnSOD)-based antioxidant protection and viability were assessed after 10 min or 30 min 1 mM hydrogen peroxide. Sham controls were kept at the same time in identical cell culture incubators. Results The PEMF increased the MnSOD-based antioxidant protection and reduced the ROS production in response to a pro-oxidant challenge. Conclusions Our work might lay foundation for the development of non-invasive PEMF-based approaches aimed at elevating endogenous antioxidant properties in cellular or tissue models.
KEYWORDS: ELF-EMF; SK-N-BE(2) cells; oxidative stimulus; reactive oxygen species; superoxide dismutase
PMID: 26940444 [PubMed - as supplied by publisher]