Author: Fan W1, Qian F1, Ma Q2, Zhang P1, Chen T1, Chen C2, Zhang Y1, Deng P2, Zhou Z2, Yu Z2.
Affiliation: 1Department of Hematology, Southwest Hospital, Third Military Medical University No. 30 Gaotanyan Avenue, Chongqing 400038, China. 2Department of Occupational Hygiene, College of Military Preventive Medicine, Third Military Medical University Chongqing 400038, China.
Conference/Journal: Int J Clin Exp Med.
Date published: 2015 May 15
Other:
Volume ID: 8 , Issue ID: 5 , Pages: 7394-404 , Word Count: 260
OBJECTIVE:
To investigate the effects of extremely low frequency electromagnetic field (ELF-EMF) on the proliferation and cytokine production of mesenchymal stem cells (MSC) and the effects of mesenchymal stem cell conditioned medium (MSC-CM) on the proliferation and migration of macrophagocytes (RAW264.7).
METHODS:
Bone marrow derived-mesenchymal stem cells (rBMSC) were isolated from rats, cultured and randomly divided into two groups: SHAM group (absence of electromagnetic field exposure) and EMF group. Cells in EMF group were exposed to ELF-EMF (50 Hz, 1 mT, 4 h/d) under sXc-ELF. Mouse mesenchymal stem cells (mMSC) were exposed to EMF for 3 days.
RESULTS:
The cell viability, DNA synthesis and proportion of cells in S phase in EMF group increased markedly when compared with SHAM group (P<0.05). When compared with SHAM group, the mRNA expressions of M-CSF and SCF increased markedly at 2 days after EMF exposure (P<0.05), the mRNA expressions of SCF, M-CSF, TPO, LIF, IL-11 and IL-7 increased dramatically, but the mRNA expressions of IL-6, SDF-1, IFN-γ and TNF-α remained unchanged (P>0.05) in mMSCs at 3 days after EMF exposure. In EMF group, the viability of RAW264.7 after MSC-CM treatment increased markedly as compared to SHAM group (P<0.05), and the ability to migrate of RAW264.7 after MSC-CM treatment in EMF group also increased significantly when compared with SHAM group (P<0.05).
CONCLUSION:
EMF is able to promote the proliferation of rBMSCs, up-regulate the expressions of hematopoietic growth factors in rBMSC and mMSC and increase the mMSC induced proliferation and migration of RAW264.7.
KEYWORDS:
Electromagnetic field; cell proliferation; cytokines; mesenchymal stem cells
PMID: 26221281 [PubMed] PMCID: PMC4509226