Author: Jung YJ1, Kim R2, Ham HJ1, Park SI1, Lee MY3, Kim J4, Hwang J5, Park MS1, Yoo SS6, Maeng LS1, Chang W7, Chung YA8.
Affiliation: 1Institute of Catholic Integrative Medicine, Incheon St. Mary's Hospital, College of Medicine, Catholic University of Korea, Incheon, Republic of Korea. 2Department of Biology Education, College of Education, Pusan National University, Busan, Republic of Korea. 3Department of Molecular Physiology, College of Pharmacy, Kyungpook National University, Daegu, Republic of Korea. 4Department of Life Systems, Sookmyung Women's University, Yongsan-gu Seoul, Republic of Korea. 5Department of Microbiology, College of Natural Science, Pusan National University, Busan, Republic of Korea. 6Department of Radiology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA. 7Department of Biology Education, College of Education, Pusan National University, Busan, Republic of Korea. Electronic address: wchang1975@pusan.ac.kr. 8Institute of Catholic Integrative Medicine, Incheon St. Mary's Hospital, College of Medicine, Catholic University of Korea, Incheon, Republic of Korea. Electronic address: nm@catholic.ac.kr.
Conference/Journal: Ultrasound Med Biol.
Date published: 2015 Apr
Other:
Volume ID: 41 , Issue ID: 4 , Pages: 999-1007 , Special Notes: doi: 10.1016/j.ultrasmedbio.2014.11.008 , Word Count: 183
A number of studies have reported the therapeutic potential of low-intensity pulsed ultrasound (LIPUS) for induction of bone repair. This study investigated whether bone regeneration might be enhanced by application of focused LIPUS to selectively stimulate fractured calvarial bone. To accomplish this, bone defects were surgically created in the middle of the skull of rats that were subsequently exposed to focused LIPUS. Bone regeneration was assessed by repeated computed tomography imaging after the operation, as well as histologic analysis with calcein, hematoxylin and eosin and proliferating cell nuclear antigen assay. At 6 wk after surgery, bone formation in the focused LIPUS-treated group improved significantly relative to the control. Interestingly, new bone tissue sprouted from focused LIPUS target points. Histologic analysis after exposure to focused LIPUS revealed that proliferating cells were significantly increased relative to the control. Taken together, these results suggest that focused LIPUS can improve re-ossification through enhancement of cell proliferation in calvarial defect sites.
Copyright © 2015 World Federation for Ultrasound in Medicine & Biology. Published by Elsevier Inc. All rights reserved.
KEYWORDS:
Bone regeneration; Calvarial bone fracture; Focused low-intensity pulsed ultrasound; Osteocyte proliferation
PMID: 25701528