Observations on the effect of the emitted qi on the reversion of nasopharyngeal carcinoma cell line

To explore a new way of treatment of nasopharngeal carcinoma by traditional Chinese medicine, we have investigated the possibility of reversing the malignancy of human poorly-differentiated squamous nasopharyngeal carcinoma cell line (CNE-2) by the emitted qi by means of double-blinded. The experiment was carried out in a way of dividing the cases into three groups: control group, treatment group and imitation group. With indices including trypan-bluestaining resistance. 3H-Thymidine infiltration, culturing in soft -agar and agglutination by PHA techniques, we elucidated the influences of the emitted qi upon the growth rate, DNA synthesis, anchorage-independent growth, and agglutination of cells. The experiment was done twice. Results show that the emitted qi can inhibit the cell proliferation. The inhibitory rates in the treatment group and imitation group were 46.0% and 9.0% in the first experiment and 48.1% and 7.6% in the second experiment respectively. The emitted qi also checked the cellular DNA synthesis, with inhibitory rates in both the above-mentioned groups being 43.0% and 7.1% in the first experiment and 45.5% and 8.2% in the second. The difference between the treatment and control groups was significant whereas that between the imitation and control group was not. In soft-agar culturing, cells treated by the emitted qi couldn't form colony and gradually became dead while cells in other two groups did form colonies. In the presence of PHA, treated cells had no obvious agglutination. But in other two groups, cells could agglutinate apparently. All evidence illustrates the fact that the emitted qi has the reversing effect on the malignancies and DNA synthesis has inhibition of the nasopharyngeal carcinoma cells. The mechanism of such an effect needs further investigation.