Anemia is the main complication of severe burn. A large amount of evidence shows that anemia can occur in several hours postburn. Effectively correcting anemia can markedly improve patient's body resistance, promote the healing of surface of wound and increase the survival rate. Moreover, new findings suggested that emitted qi could promote the amount of RBC and the concentrations of hemoglobin. However,no reports about the function of emitted qi on burnt patient's concentrations of hemoglobin have been found, so this paper aims to investigate the effect of emitted qi on burnt rat's concentration of hemoglobin, and provides experimental evidence for qigong treatment on burn.
Materials and Methods:
1.1 Male Wistar rats were randomly divided into a burnt control group and a burnt-treating group.
1.2 Mode of burn: Depilatory parts of rats were immersed into boiling water for 15 min. burn injury was 25% TBSA, full skin thickness.
1.3 The treating group received emitted qi from a qigong master 30 min once a day for 14 days postburn.
1.4 Observing index: The concentration of hemoglobin in rats were determined on day 1,3,7,10,14 and 21 postburn.
1.5 The differences between the two groups were statistically analysed by using student's t-test.
Results:
The effect of emitted qi on the concentration of hemoglobin in burnt rats was summarized in Table I. Of both the groups examined in this study, hemoglobin (Hb) in the control group got to the lowest point on day 1 postburn,(p<0.01) markedly lower than preburn and that in the treating group. While Hb in the treating group was significantly lower than preburn level, but higher than the control group on day 3 postburn. On day 7 postburn, no difference of Hb in the treating group and in preburn was found, while,not any significance of Hb between the control group and preburn appeared on day 14 postburn.

Table I-Effect of emitted qi on the concentration of Hb
group preburn postburn (day)
1 3 7 10 14 21
control 11.08± 7.22± 8.12± 9.82± 9.27± 10.55± 11.70±
group(n=12) 0.81 1.10** 1.09** 1.03* 0.84* 1.45 0.81
treating 1.18± 10.67± 9.56± 10.43± 10.12± 10.42± 11.42±
group(n=12) 1.16 1.35@@ 0.89@@ 0.77 1.09@ 1.13 0.61

± indicates +/- for standard deviation
*: p<0.01 **: p<0.01
@:p<0.01 @@: p<0.01

Discussion:
Our results demonstrated that the concentration of Hb in the control group declined to the lowest level on day 1 postburn,while the concentration of Hb in the treating group got to the lowest point on day 3 postburn, which was higher than that of the control group. This showed that emitted qi might have some protective effect on red blood cells following thermal injury. Kong et al., reported that emitted qi could markedly reduce 'blood stasis' of model animal's RBC fragility, promote RBC membrane fluidity and the activity of ATPse. Our previous findings have already pointed to the facts that emitted qi could decrease the mice RBC fragility. The fact above demonstrated that emitted qi had a protective effect on injury RBC.
The concentrations of hemoglobin in the treating group returned to normal value 7 days earlier than that in the control. This evidence indicated that emitted qi not only affects on protective RBC, but promotes synthesis of hemoglobin.

EFFECTS OF EMITTED QI ON THE CONCENTRATIONS OF HEMOGLOBIN IN BURNT RATS