Human Skin Fibroblast Telomeres are Shortened after Ultraviolet Irradiation. 


Author: Ma HM, Liu W, Zhang P, Yuan XY.

Affiliation: Department of Dermatology, First Affiliated Hospital of China Medical University Hospital One, Shenyang, Liaoning, China. 
Conference/Journal: J Int Med Res.
Date published: 2012
Other:
 Volume ID: 40   ,  Issue ID: 5   ,  Pages: 1871-7   ,  Word Count: 155  
    OBJECTIVE:

Telomere length was used as a biomarker of cell senescence to explore the role of telomere shortening in photoageing induced by ultraviolet A (UVA) light.

METHODS:

Real-time polymerase chain reaction was used to determine telomere length in cultured human fibroblasts of different generations and after exposure to UVA at doses up to 10 000 mJ/cm(2). Twoway analysis of variance was used to determine whether passaging or UVA was the main factor contributing to telomere shortening.

RESULTS:

In nonirradiated cells, telomere length was inversely related to cell generation number. In fibroblasts exposed to UVA at a dose of 1000 or 10 000 mJ/cm(2), telomere length was significantly shorter than that of nonirradiated controls and was negatively related to UVA dose.

CONCLUSIONS:

Telomere length and subsequent cell viability may be affected by UVA irradiation. DNA damage caused by UVA irradiation may initiate the photoageing process and telomeres may be a useful new target for attempts to prevent photoageing.

PMID: 23206469