Low-Intensity Pulsed Ultrasound Enhances BMP-7-Induced Osteogenic Differentiation of Human Fracture Hematoma-Derived Progenitor Cells In Vitro. 


Author: Lee SY, Koh A, Niikura T, Oe K, Koga T, Dogaki Y, Kurosaka M.

Affiliation: Department of Orthopaedic Surgery, Kobe University Graduate School of Medicine, Kobe, Japan. 
Conference/Journal: J Orthop Trauma.
Date published: 2012 Apr 28
Other:
  Word Count: 184  
    OBJECTIVE:

To investigate the effect of the combined application of bone morphogenetic protein-7 (BMP-7) and low-intensity pulsed ultrasound (LIPUS) on human fracture hematoma-derived progenitor cells (HCs).

METHODS:

HCs were isolated from 6 patients. The cells were then divided into 4 groups and cultured: (1) control group, HCs cultured in growth medium without LIPUS; (2) LIPUS group, HCs cultured in growth medium with LIPUS; (3) BMP-7 group, HCs cultured in osteogenic medium containing BMP-7 without LIPUS; and (4) BMP-7 + LIPUS group, HCs cultured in osteogenic medium with LIPUS. Osteogenic differentiation potential and proliferation of HCs were compared among 4 groups.

RESULTS:

Alkaline phosphatase activity, the expression of osteogenic genes, and the mineralization of HCs in BMP-7 + LIPUS group were shown to be significantly increased compared with the other groups. However, LIPUS did not affect the proliferation of HCs in the presence or absence of BMP-7.

CONCLUSIONS:

These findings demonstrated for the first time the significant effect of LIPUS on the osteogenic differentiation of HCs in the presence of BMP-7. This study may provide significant evidence for the clinical combined application of BMP-7 and LIPUS for the treatment of acute bone fractures.

PMID: 22549031