External Qi of Yan Xin Qigong Induces Apoptosis and Inhibits Migration and Invasion of Estrogen-Independen t Breast Cancer Cells Through Suppression of Akt/NF-kB Signaling.


Author: Yan X, Shen H, Jiang H, Hu D, Zhang C, Wang J, Wu X. 

Affiliation: hongqing Institute of Traditional Chinese Medicine, Chongqing.
Conference/Journal: Cell Physiol Biochem.
Date published: 2010
Other:
 Volume ID: 25   ,  Issue ID: 2-3   ,  Pages: 263-270   ,  Word Count: 143  
    The antitumor effects of external Qi of Yan Xin Qigong

(YXQ-EQ) have been widely described over the past three decades. To gain a

better understanding of the mechanisms underlying YXQ-EQ\'s antitumor effects,

in the present study we investigated its effects on growth, migration, invasion

and apoptosis of breast cancer cells and the underlying molecular mechanisms.

We show that YXQ-EQ treatment caused a time-dependent reduction in viability,

blocked clonogenic growth and induced apoptosis in estrogen-independen t breast

cancer MDA-MB-231 cells. Furthermore, YXQ-EQ treatment blocked migration and

invasion of MDA-MB-231 cells. Biochemically, YXQ-EQ treatment markedly

inhibited constitutive and EGF-induced Akt phosphorylation. YXQ-EQ also

substantially repressed NF-kappaB activity, resulting in decreased expression

of anti-apoptotic Bcl-2, Bcl-X(L), XIAP and survivin proteins. These findings

suggest that YXQ-EQ may induce apoptosis and inhibition of migration and invasion

of MDA-MB-231 cells through the repression of Akt/NF-kappaB signaling.