Impact of mind-body intervention on proinflammatory cytokines interleukin 6 and 1β: a three-arm randomized controlled trial for persons with sleep disturbance and depression Author: Siu-Man Ng1, Margaret X C Yin2, Jessie S M Chan3, Celia H Y Chan3, Ted C T Fong4, Ang Li5, Kwok-Fai So5, Lai-Ping Yuen6, Jian-Ping Chen7, Ka-Fai Chung8, Cecilia L W Chan3 Affiliation: <sup>1</sup> Departments of Social Work and Social Administration, The University of Hong Kong, Hong Kong SAR; Centre on Behavioral Health, Faculty of Social Science, The University of Hong Kong, Hong Kong SAR. Electronic address: ngsiuman@hku.hk. <sup>2</sup> Departments of Social Work and Social Administration, The University of Hong Kong, Hong Kong SAR. <sup>3</sup> Departments of Social Work and Social Administration, The University of Hong Kong, Hong Kong SAR; Centre on Behavioral Health, Faculty of Social Science, The University of Hong Kong, Hong Kong SAR. <sup>4</sup> Centre on Behavioral Health, Faculty of Social Science, The University of Hong Kong, Hong Kong SAR. <sup>5</sup> Guangdong-Hong Kong-Macau Institute of CNS Regeneration, Guangdong Key Laboratory of Brain Function and Diseases, Jinan University, Guangzhou 510632, China; Departments of Ophthalmology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR; State Key Laboratory of Brain and Cognitive Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR. <sup>6</sup> International Association for Health and Yangsheng, Hong Kong, Hong Kong SAR. <sup>7</sup> School of Chinese medicine, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR. <sup>8</sup> Department of Psychiatry, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong SAR. Conference/Journal: Brain Behav Immun Date published: 2021 Oct 8 Other: Special Notes: doi: 10.1016/j.bbi.2021.09.022. , Word Count: 304 Depressed people are prone to sleep disturbance, which may in return perpetuate the depression. Both depression and sleep disturbance influence proinflammatory cytokines interleukin (IL) 6 and 1β. Thus interventions for depression should consider the effect on sleep disturbance, and vice versa. Integrative Body-Mind-Spirit (IBMS) and Qigong interventions have been applied in a wide range of health and mental health conditions, including depression and sleep disturbance. This study aimed to evaluate the effect of these two mind-body therapies for persons with both depressive symptoms and sleep disturbance. A three-arm randomized controlled trial was conducted among 281 participants, who were randomly assigned to either IBMS, Qigong or wait list control group. Participants in IBMS and Qigong groups received eight weekly sessions of intervention. Outcome measures were plasma concentrations of IL-6 and IL-1β, and a questionnaire containing Pittsburgh Sleep Quality Index, Center for Epidemiologic Studies Depression Scale, Somatic Symptom Inventory, Perceived Stress Scale and Body-Mind-Spirit Holistic Well-being Scale. Outcomes were assessed at baseline (T0), immediate post-intervention (T1) and at three-months post-intervention (T2). Besides intervention efficacy analysis, path analysis was performed to explore the relations among perceived stress, depression, sleep disturbance, and IL-6 and IL-1β values. The study found both IBMS and Qigong reduced depression, sleep disturbance, painful and painless somatic symptoms, IL-6 and IL-1β levels, and increased holistic well-being. The effect sizes of IBMS and Qigong, mostly in the medium magnitude range, were approximatively equivalent. Path analysis models revealed a predictive role of perceived stress in depression and sleep disturbance, a bidirectional relationship between depression and sleep disturbance, and significant influence of depression and sleep disturbance on IL-6 and IL-1β. Compared with control, the findings support the efficacy of IBMS and Qigong interventions in relieving depression and sleep disturbance, and in reducing IL-6 and IL-1β levels. Keywords: Depression; Integrative Body-Mind-Spirit (IBMS); Interleukin-1β; Interleukin-6; Qigong; Sleep disturbance. PMID: 34634445 DOI: 10.1016/j.bbi.2021.09.022