Transcriptional Changes in Cancer Cells Induced by Exposure to a Healing Method. Author: Beseme S1,2, Bengston W3, Radin D4, Turner M5, McMichael J1,2 Affiliation: <sup>1</sup>Beech Tree Labs, Inc, Providence, RI, USA. <sup>2</sup>The Institute for Therapeutic Discovery, Delanson, NY, USA. <sup>3</sup>St Joseph's College, Patchogue, NY, USA. <sup>4</sup>Institute of Noetic Sciences (IONS), Petaluma, CA, USA. <sup>5</sup>Institute of Electrical and Electronic Engineers, MDT Consulting, Huntsville, AL, USA. Conference/Journal: Dose Response. Date published: 2018 Jul 11 Other: Volume ID: 16 , Issue ID: 3 , Pages: 1559325818782843 , Special Notes: doi: 10.1177/1559325818782843. eCollection 2018 Jul-Sep. , Word Count: 207 Energy healing, or healing with intent, is a complementary and alternative medicine therapy reported to be beneficial with a wide variety of conditions. We are developing a delivery technology for a method previously tested in mouse models with solid tumors (the Bengston method) independent of the presence of a healer. The goal of this study was to assess whether stored or recorded energy has an impact on breast cancer cells in vitro, using energy-charged cotton and electromagnetic recording of healers practicing the method. Expression of genes involved in cancer and inflammation pathways was measured by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Treatment of cells using energy-charged cotton resulted in statistically significant changes <1.5-fold. In cells exposed to an electromagnetic recording, 37 genes of 167 tested showed a >1.5-fold change when compared to the control, and 68 genes showing statistically significant fold changes. Two genes, ATP citrate lyase (ACLY) and interleukin 1β (IL-1β), were consistently downregulated at 4 and 24 hours of exposure to the recording, respectively, in 3 independent experiments. Both ACLY and IL-1β were also downregulated in cells exposed to a hands-on delivery of the method, suggesting these 2 genes as potential markers of the healing method. KEYWORDS: audio; breast cancer; complementary and alternative medicine; energy medicine PMID: 30022894 PMCID: PMC6047252 DOI: 10.1177/1559325818782843