Schwann-like cells differentiated from human dental pulp stem cells combined with a pulsed electromagnetic field can improve peripheral nerve regeneration.


Author: Hei WH1,2, Kim S3, Park JC4, Seo YK5, Kim SM1, Jahng JW6, Lee JH1,6.

Affiliation: 1Department of Oral and Maxillofacial Surgery, School of Dentistry, Seoul National University, Seoul, Korea. 2Key Laboratory of Oral Biomedical Engineering of Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, China. 3Graduate School of Bio and Information Technology, Hankyong National University, Anseong-si, Kyonggi-do, Seoul, Korea. 4Department of Oral Histology-Developmental Biology, School of Dentistry, Seoul National University, Seoul, Korea. 5Dongguk Department of Medical Biotechnology, College of Life Science and Biotechnology, Dongguk University, Seoul, Korea. 6Dental Research Institute, Seoul National University, Seoul, Korea.
Conference/Journal: Bioelectromagnetics.
Date published: 2016 Mar 15
Other:
  Special Notes: doi: 10.1002/bem.21966. [Epub ahead of print]   ,  Word Count: 216  
    The purpose of this study was to investigate the effect of Schwann-like cells combined with pulsed electromagnetic field (PEMF) on peripheral nerve regeneration. Schwann-like cells were derived from human dental pulp stem cells (hDPSCs) and verified with CD104, S100, glial fibrillary acidic protein (GFAP), laminin, and P75NTR immunocytochemistry. Gene expression of P75NTR and S100 were analyzed. Male Sprague-Dawley rats (200-250g, 6-week-old) were divided into seven groups (n = 10 each): control, sham, PEMF, hDPSCs, hDPSCs + PEMF, Schwann-like cells, Schwann-like cells + PEMF. Cells were transplanted (1 × 106 /10µl/rat) at crush-injury site or combined with PEMF (50 Hz, 1 h/day, 1 mT). Nerve regeneration was evaluated with functional test, histomorphometry and retrograde labelled neurons. Schwann-like cells expressed CD104, S100, GFAP, laminin, and p75 neurotrophin receptor (P75NTR ). P75NTR and S100 mRNA expression was highest in Schwann-like cells + PEMF group, which also showed increased Difference and Gap scores. Axons and retrograde labeled neurons increased in all treatment groups. Schwann-like cells, hDPSCs with or without PEMF, and PEMF only improved peripheral nerve regeneration. Schwann-like cells + PEMF showed highest regeneration ability; PEMF has additive effect on hDPSCs, Schwann-like cell in vitro and nerve regeneration ability after transplantation in vivo. Bioelectromagnetics. © 2016 Wiley Periodicals, Inc.



© 2016 Wiley Periodicals, Inc.



KEYWORDS: CD104; GFAP; P75NTR; S100; crush nerve injury



PMID: 26991921  [PubMed - as supplied by publisher]