Metabonomic study of the effects of different acupuncture directions on therapeutic efficacy.


Author: Ju L1, Wen Y2, Yin J1, Deng S2, Zheng J2, Wang L1, Deng H1, Hou Z1, Zhao X2, He S2, Huang L2, Zhang C2, Tian G2, Meng Z3, Li Y4.

Affiliation: 1Tianjin University of Traditional Chinese Medicine, 312 Anshan West Road, Tianjin 300193, China. 2Department of Acupuncture and Moxibustion, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, No. 314, West Anshan Avenue, Tianjin 300193, China. 3Department of Acupuncture and Moxibustion, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine, No. 314, West Anshan Avenue, Tianjin 300193, China. Electronic address: tjmzh86@163.com. 4Tianjin University of Traditional Chinese Medicine, 312 Anshan West Road, Tianjin 300193, China. Electronic address: yuboli1@163.com.
Conference/Journal: J Chromatogr B Analyt Technol Biomed Life Sci.
Date published: 2015 Dec 8
Other:
 Volume ID: 1009-1010   ,  Pages: 87-95   ,  Special Notes: doi: 10.1016/j.jchromb.2015.12.006.   ,  Word Count: 205  
    Abstract

Posterior circulation ischemia (PCI) is a common clinical ischemic cerebrovascular disease that can endanger the lives of patients in severe cases. Our previous research found that needling the Fengchi (GB20) acupoint presents a significant effect on PCI and that different acupuncture directions can exert different effects. To investigate the biological mechanism of acupuncture directions, rapid resolution liquid chromatography coupled with quadrupole time-of-flight mass spectrometry-based metabonomic techniques are used to analyze the metabolic profiles of urine samples. The urine samples were obtained from 30 healthy control subjects, 60 PCI patients before and after treatment of different acupuncture directions. Six metabolites, including LPE (22:6), estrone, uric acid, vanillylmandelic acid, N-acetyl-l-tyrosine, and 4-hydroxyphenylacetylglutamine were identified as potential biomarkers of acupuncture treatment of PCI. Acupuncture treatment of PCI patients significantly changed the levels of these potential biomarkers. Moreover, different acupuncture directions showed different effects on the contents of these biomarkers. These results strongly support the belief that acupuncture direction performs an important function in acupuncture intervention. The findings provide new insights into the mechanism of acupuncture treatment and reveal that acupuncture manipulation results in various curative.

Copyright © 2015 Elsevier B.V. All rights reserved.

KEYWORDS:

Acupuncture; Metabonomics; metabolomics; Posterior circulation ischemia; Rapid resolution liquid chromatography coupled with quadrupole time-of-flight mass spectrometry

PMID: 26708629